Immunological imprint of COVID-19 on human peripheral blood leukocyte populations.
B cells
SARS-CoV-2
T cells
clinical immunology
coronavirus disease 2019
flow cytometry
infections
lymphocytes
Journal
Allergy
ISSN: 1398-9995
Titre abrégé: Allergy
Pays: Denmark
ID NLM: 7804028
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
18
09
2020
revised:
12
10
2020
accepted:
14
10
2020
pubmed:
1
11
2020
medline:
19
3
2021
entrez:
31
10
2020
Statut:
ppublish
Résumé
SARS-CoV-2 has triggered a pandemic that is now claiming many lives. Several studies have investigated cellular immune responses in COVID-19-infected patients during disease but little is known regarding a possible protracted impact of COVID-19 on the adaptive and innate immune system in COVID-19 convalescent patients. We used multiparametric flow cytometry to analyze whole peripheral blood samples and determined SARS-CoV-2-specific antibody levels against the S-protein, its RBD-subunit, and viral nucleocapsid in a cohort of COVID-19 convalescent patients who had mild disease ~10 weeks after infection (n = 109) and healthy control subjects (n = 98). Furthermore, we correlated immunological changes with clinical and demographic parameters. Even ten weeks after disease COVID-19 convalescent patients had fewer neutrophils, while their cytotoxic CD8 Acute SARS-CoV-2 infection leaves protracted beneficial (ie, activation of T cells) and potentially harmful (ie, reduction of neutrophils) imprints in the cellular immune system in addition to induction of specific antibody responses.
Sections du résumé
BACKGROUND
SARS-CoV-2 has triggered a pandemic that is now claiming many lives. Several studies have investigated cellular immune responses in COVID-19-infected patients during disease but little is known regarding a possible protracted impact of COVID-19 on the adaptive and innate immune system in COVID-19 convalescent patients.
METHODS
We used multiparametric flow cytometry to analyze whole peripheral blood samples and determined SARS-CoV-2-specific antibody levels against the S-protein, its RBD-subunit, and viral nucleocapsid in a cohort of COVID-19 convalescent patients who had mild disease ~10 weeks after infection (n = 109) and healthy control subjects (n = 98). Furthermore, we correlated immunological changes with clinical and demographic parameters.
RESULTS
Even ten weeks after disease COVID-19 convalescent patients had fewer neutrophils, while their cytotoxic CD8
CONCLUSION
Acute SARS-CoV-2 infection leaves protracted beneficial (ie, activation of T cells) and potentially harmful (ie, reduction of neutrophils) imprints in the cellular immune system in addition to induction of specific antibody responses.
Identifiants
pubmed: 33128792
doi: 10.1111/all.14647
pmc: PMC7984452
doi:
Substances chimiques
Antibodies, Viral
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
751-765Subventions
Organisme : Österreichische Forschungsförderungsgesellschaft
ID : 35721032
Organisme : Austrian Science Fund
ID : DK-W1248
Organisme : Viravaxx
Organisme : Medizinisch-Wissenschaftlichr Fonds des Bürgermeisters der Bundeshauptstadt Wien
ID : COVID001
Organisme : Medizinisch-Wissenschaftlichr Fonds des Bürgermeisters der Bundeshauptstadt Wien
ID : COVID006
Informations de copyright
© 2020 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.
Références
J Immunol. 1994 Aug 1;153(3):952-9
pubmed: 7913116
Am J Med Sci. 1946 Aug;212(2):129-38
pubmed: 20996954
Arch Virol. 2009;154(7):1093-9
pubmed: 19526193
Lab Chip. 2015 Mar 21;15(6):1574-89
pubmed: 25648429
Clin Infect Dis. 2020 Nov 5;71(8):1937-1942
pubmed: 32301997
EBioMedicine. 2020 Jul;57:102885
pubmed: 32650275
Proc Natl Acad Sci U S A. 2020 May 19;117(20):10970-10975
pubmed: 32350134
Lancet. 2020 Feb 15;395(10223):497-506
pubmed: 31986264
Eur J Immunol. 2019 Oct;49(10):1457-1973
pubmed: 31633216
Allergy. 2020 Jul;75(7):1730-1741
pubmed: 32077115
Clin Chem. 2020 Nov 1;66(11):1405-1413
pubmed: 32777031
Science. 1996 Apr 5;272(5258):54-60
pubmed: 8600537
Nat Immunol. 2013 Apr;14(4):356-63
pubmed: 23475183
J Infect Dis. 2020 May 11;221(11):1762-1769
pubmed: 32227123
J Exp Med. 1979 Aug 1;150(2):246-55
pubmed: 88499
Lancet. 2020 Feb 15;395(10223):470-473
pubmed: 31986257
Allergy. 2021 Mar;76(3):878-883
pubmed: 32734595
Allergy. 2020 Jul;75(7):1742-1752
pubmed: 32239761
Cell. 2020 Oct 1;183(1):158-168.e14
pubmed: 32979941
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
Am J Med. 1984 Jan;76(1):85-90
pubmed: 6318560
J Trop Pediatr. 1991 Mar;37(2):67-70
pubmed: 2027167
Int J Hematol. 2020 Oct;112(4):553-559
pubmed: 32656638
J Immunol. 1993 Apr 1;150(7):3070-9
pubmed: 8454874
Nature. 2003 Nov 27;426(6965):450-4
pubmed: 14647384
Clin Infect Dis. 2020 Jul 28;71(15):762-768
pubmed: 32161940
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15748-53
pubmed: 15496474
Immunity. 2016 Jun 21;44(6):1379-91
pubmed: 27287409
AIDS. 1992 Aug;6(8):793-7
pubmed: 1418775
Crit Care. 2020 Apr 30;24(1):188
pubmed: 32354360
J Immunol. 1998 Jan 1;160(1):395-402
pubmed: 9551996
J Infect. 2020 Jul;81(1):e51-e60
pubmed: 32315725
Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):14857-14863
pubmed: 32527856
Allergy. 2021 Feb;76(2):574-577
pubmed: 32754908
Allergy. 2020 Oct;75(10):2503-2541
pubmed: 32535955
J Virol. 2020 Mar 17;94(7):
pubmed: 31996437
J Med Virol. 2020 Nov;92(11):2260-2262
pubmed: 32462717
Br J Haematol. 2020 Jul;190(1):33-36
pubmed: 32420610
Hum Immunol. 2004 Apr;65(4):282-90
pubmed: 15120183
Cytometry A. 2008 Nov;73(11):975-83
pubmed: 18785267
Eur J Immunol. 1995 May;25(5):1477-80
pubmed: 7774653
Science. 2020 Sep 4;369(6508):
pubmed: 32669297
Nat Commun. 2020 Jul 8;11(1):3410
pubmed: 32641700
Blood. 2009 Mar 12;113(11):2461-9
pubmed: 18987362
Nat Commun. 2018 Jun 18;9(1):2382
pubmed: 29915220
Lancet. 1956 Jul 14;271(6933):66-8
pubmed: 13347088
Am J Trop Med Hyg. 1976 May;25(3):456-62
pubmed: 180844
Pediatr Infect Dis J. 1988 Jun;7(6):429-30
pubmed: 2839816
Allergy. 2020 Jul;75(7):1564-1581
pubmed: 32396996
Pediatr Rev. 2008 Jan;29(1):12-23; quiz 24
pubmed: 18166617
Sci Immunol. 2020 Jul 15;5(49):
pubmed: 32669287
Allergy. 2020 Jul;75(7):1809-1812
pubmed: 32281110
Front Cell Infect Microbiol. 2020 Jun 30;10:364
pubmed: 32695683
Circulation. 2005 May 24;111(20):2605-10
pubmed: 15897343
Adv Exp Med Biol. 2006;581:265-9
pubmed: 17037540
Stem Cell Rev Rep. 2020 Jun;16(3):434-440
pubmed: 32307653
Int J Infect Dis. 2020 Sep;98:353-358
pubmed: 32634585
Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6776-81
pubmed: 17428922
Cytometry B Clin Cytom. 2014 May;86(3):191-206
pubmed: 24488780
Nat Immunol. 2020 Nov;21(11):1336-1345
pubmed: 32887977
Front Immunol. 2020 Aug 07;11:1949
pubmed: 32849654
Allergy. 2021 Mar;76(3):751-765
pubmed: 33128792
Cell. 2020 Apr 16;181(2):223-227
pubmed: 32220310
Allergy. 2017 May;72(5):754-763
pubmed: 27753449
Clin Exp Immunol. 2001 Sep;125(3):485-91
pubmed: 11531958