Ameliorating effects of curcumin-loaded superparamagnetic iron oxide nanoparticles (SPIONs) on the mouse testis exposed to the transient hyperthermia: A molecular and stereological study.
Animals
Antioxidants
/ pharmacokinetics
Cell Survival
/ drug effects
Curcumin
/ pharmacokinetics
DNA Fragmentation
/ drug effects
Drug Carriers
Gene Expression
Heat-Shock Response
/ drug effects
Hyperthermia
/ drug therapy
Interleukin-1alpha
/ genetics
Interleukin-6
/ genetics
Leydig Cells
/ drug effects
Magnetic Iron Oxide Nanoparticles
/ administration & dosage
Male
Mice
Oxidative Stress
/ drug effects
Protective Agents
/ pharmacokinetics
Scrotum
/ drug effects
Sperm Count
Sperm Motility
/ drug effects
Spermatogenesis
/ drug effects
Spermatozoa
/ drug effects
Testis
/ drug effects
Tumor Necrosis Factor-alpha
/ genetics
Curcumin-loaded SPIONs
Hyperthermia
Testes
Journal
Acta histochemica
ISSN: 1618-0372
Titre abrégé: Acta Histochem
Pays: Germany
ID NLM: 0370320
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
08
07
2020
revised:
20
09
2020
accepted:
21
09
2020
pubmed:
1
11
2020
medline:
31
8
2021
entrez:
31
10
2020
Statut:
ppublish
Résumé
Testicular hyperthermia can have negative effects on male fertility. Despite reported therapeutic benefits of curcumin, several factors often limit its application such as low water solubility and instable structure. Curcumin-loaded superparamagnetic iron oxide nanoparticles (SPIONs) were designed to solve its limitation of use. In the present study, we evaluated the effect of curcumin-loaded SPIONs on transient testicular hyperthermia in mouse. A total of 18 adult male NMRI mice were divided into three groups (n = 6): I. Controls (Cont), II. Scrotal hyperthermia (Hyp), III. Scrotal hyperthermia + curcumin-loaded iron particles (240 μL) (Hyp + Cur). After seventy days, the animals were sacrificed and used for further molecular and stereological evaluations. Sperm count, motility and viability significantly decreased in group hyp as compared to cont group. Furthermore, Sperm DNA fragmentation and cell apoptosis in testes increased remarkably in group hyp, compared with group cont. Stereological study showed a reduction in number of spermatogenic and Leydig cells, as well as reduced weight and volume of testes in hyp group. Degenerative appearance of testes exposed to hyperthermia was also observed. In addition, higher mRNA expression of inflammatory cytokines (IL1-α, IL6, and TNF-α) was detected in group hyp compared to cont group. However, curcumin-loaded SPIONs alleviated all of the pathologic changes in the Hyp + Cur group compared to the hyp group. Here, we used nanoparticle form of curcumin in testicular hyperthermia model and showed its ameliorating effects on testes damages caused by heat stress, which can be an appropriate method to overcome the problems that limit curcumin application in cases with increased intra testicular temperature.
Sections du résumé
BACKGROUND
BACKGROUND
Testicular hyperthermia can have negative effects on male fertility. Despite reported therapeutic benefits of curcumin, several factors often limit its application such as low water solubility and instable structure. Curcumin-loaded superparamagnetic iron oxide nanoparticles (SPIONs) were designed to solve its limitation of use. In the present study, we evaluated the effect of curcumin-loaded SPIONs on transient testicular hyperthermia in mouse.
MATERIALS AND METHOD
METHODS
A total of 18 adult male NMRI mice were divided into three groups (n = 6): I. Controls (Cont), II. Scrotal hyperthermia (Hyp), III. Scrotal hyperthermia + curcumin-loaded iron particles (240 μL) (Hyp + Cur). After seventy days, the animals were sacrificed and used for further molecular and stereological evaluations.
RESULTS
RESULTS
Sperm count, motility and viability significantly decreased in group hyp as compared to cont group. Furthermore, Sperm DNA fragmentation and cell apoptosis in testes increased remarkably in group hyp, compared with group cont. Stereological study showed a reduction in number of spermatogenic and Leydig cells, as well as reduced weight and volume of testes in hyp group. Degenerative appearance of testes exposed to hyperthermia was also observed. In addition, higher mRNA expression of inflammatory cytokines (IL1-α, IL6, and TNF-α) was detected in group hyp compared to cont group. However, curcumin-loaded SPIONs alleviated all of the pathologic changes in the Hyp + Cur group compared to the hyp group.
CONCLUSION
CONCLUSIONS
Here, we used nanoparticle form of curcumin in testicular hyperthermia model and showed its ameliorating effects on testes damages caused by heat stress, which can be an appropriate method to overcome the problems that limit curcumin application in cases with increased intra testicular temperature.
Identifiants
pubmed: 33128988
pii: S0065-1281(20)30131-8
doi: 10.1016/j.acthis.2020.151632
pii:
doi:
Substances chimiques
Antioxidants
0
Drug Carriers
0
IL6 protein, human
0
Il1a protein, mouse
0
Interleukin-1alpha
0
Interleukin-6
0
Protective Agents
0
Tumor Necrosis Factor-alpha
0
Curcumin
IT942ZTH98
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
151632Informations de copyright
Copyright © 2020. Published by Elsevier GmbH.