Residual SARS-CoV-2 RNA in nasal swabs of convalescent COVID-19 patients: Is prolonged quarantine always justified?
COVID-19
Cq value
Infectivity
Real-time reverse transcription PCR
SARS-CoV-2
Virus isolation
Journal
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
ISSN: 1878-3511
Titre abrégé: Int J Infect Dis
Pays: Canada
ID NLM: 9610933
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
21
09
2020
revised:
21
10
2020
accepted:
23
10
2020
pubmed:
2
11
2020
medline:
20
1
2021
entrez:
1
11
2020
Statut:
ppublish
Résumé
Real-time reverse transcription PCR is currently the most sensitive method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Defining whether a patient could be contagious or not contagious in the presence of residual SARS-CoV-2 RNA is of extreme importance in the context of public health. In this prospective multicenter study, virus isolation was prospectively attempted in 387 nasal swabs from clinically recovered patients showing low viral load (quantification cycle, Cq, value greater than 30). The median Cq value was 36.8 (range 30.0-39.4). Overall, a cytopathic effect was detected in nine samples, corresponding to a culture positivity rate of 2.3% (9/387). The results of this study help to dissect true virus replication and residual viral RNA detection in recovered patients.
Identifiants
pubmed: 33130202
pii: S1201-9712(20)32278-5
doi: 10.1016/j.ijid.2020.10.072
pmc: PMC7834568
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
299-302Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.
Références
Emerg Infect Dis. 2020 Oct;26(10):2353-2360
pubmed: 32723432
Clin Chem. 2009 Apr;55(4):611-22
pubmed: 19246619
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
Nature. 2020 May;581(7809):465-469
pubmed: 32235945
Lancet. 2020 Apr 25;395(10233):1339-1340
pubmed: 32304647
JAMA Netw Open. 2020 May 1;3(5):e209759
pubmed: 32442288
Eur J Clin Microbiol Infect Dis. 2020 Jun;39(6):1059-1061
pubmed: 32342252
JAMA. 2020 May 12;323(18):1843-1844
pubmed: 32159775
J Clin Microbiol. 2020 Jul 23;58(8):
pubmed: 32518072
Euro Surveill. 2020 Aug;25(32):
pubmed: 32794447