SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assemblies.
Cytoplasm
/ metabolism
Green Fluorescent Proteins
/ analysis
HEK293 Cells
Heterogeneous-Nuclear Ribonucleoprotein K
/ metabolism
Heterogeneous-Nuclear Ribonucleoproteins
/ metabolism
Humans
Peptide Chain Initiation, Translational
Polypyrimidine Tract-Binding Protein
/ metabolism
RNA, Long Noncoding
/ metabolism
RNA-Binding Proteins
/ metabolism
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
18 11 2020
18 11 2020
Historique:
accepted:
23
09
2020
revised:
26
08
2020
received:
04
02
2020
pubmed:
2
11
2020
medline:
4
2
2021
entrez:
1
11
2020
Statut:
ppublish
Résumé
SINEUPs are long non-coding RNAs (lncRNAs) that contain a SINE element, and which up-regulate the translation of target mRNA. They have been studied in a wide range of applications, as both biological and therapeutic tools, although the underpinning molecular mechanism is unclear. Here, we focused on the sub-cellular distribution of target mRNAs and SINEUP RNAs, performing co-transfection of expression vectors for these transcripts into human embryonic kidney cells (HEK293T/17), to investigate the network of translational regulation. The results showed that co-localization of target mRNAs and SINEUP RNAs in the cytoplasm was a key phenomenon. We identified PTBP1 and HNRNPK as essential RNA binding proteins. These proteins contributed to SINEUP RNA sub-cellular distribution and to assembly of translational initiation complexes, leading to enhanced target mRNA translation. These findings will promote a better understanding of the mechanisms employed by regulatory RNAs implicated in efficient protein translation.
Identifiants
pubmed: 33130894
pii: 5936032
doi: 10.1093/nar/gkaa814
pmc: PMC7672464
doi:
Substances chimiques
Heterogeneous-Nuclear Ribonucleoprotein K
0
Heterogeneous-Nuclear Ribonucleoproteins
0
PTBP1 protein, human
0
RNA, Long Noncoding
0
RNA-Binding Proteins
0
enhanced green fluorescent protein
0
Polypyrimidine Tract-Binding Protein
139076-35-0
HNRNPK protein, human
146410-60-8
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11626-11644Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
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