Interaction of sperm with endometrium can regulate genes involved in endometrial receptivity pathway in mice: An experimental study.
Gene expression
Mice
Endometrial receptivity
Journal
International journal of reproductive biomedicine
ISSN: 2476-4108
Titre abrégé: Int J Reprod Biomed
Pays: Iran
ID NLM: 101679102
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
19
05
2019
revised:
04
11
2019
accepted:
30
05
2020
entrez:
2
11
2020
pubmed:
3
11
2020
medline:
3
11
2020
Statut:
epublish
Résumé
Many researchers consider implantation and endometrial receptivity as pertinent issues in reproductive science. Although, several experiments have been performed and their results evaluated, yet there is no confirmed evidence about the related factors and the role of sperm in endometrial receptivity. To investigate the effect of the sperm-endometrium interaction in regulating genes involved in the endometrial receptivity pathway. In this experimental study, 10 male and 30 female NMRI mice were included, and half of the male cases were vasectomized. The subjects were divided into two groups as follows; group 1 (case) comprised of 15 females mated with 5 non-vasectomized male mice, while group 2 (control) consisted of 15 females mated with 5 vasectomized males. Cases were sacrificed and assessed after 36 hr and the endometrial tissue was extracted and kept at -80°C until the next use. The expression of the endometrial receptivity pathway genes, including The mRNA levels of Finally, seminal plasma was observed to be effective in expressing the involved genes in the successful implantation pathway, including
Sections du résumé
BACKGROUND
BACKGROUND
Many researchers consider implantation and endometrial receptivity as pertinent issues in reproductive science. Although, several experiments have been performed and their results evaluated, yet there is no confirmed evidence about the related factors and the role of sperm in endometrial receptivity.
OBJECTIVE
OBJECTIVE
To investigate the effect of the sperm-endometrium interaction in regulating genes involved in the endometrial receptivity pathway.
MATERIALS AND METHODS
METHODS
In this experimental study, 10 male and 30 female NMRI mice were included, and half of the male cases were vasectomized. The subjects were divided into two groups as follows; group 1 (case) comprised of 15 females mated with 5 non-vasectomized male mice, while group 2 (control) consisted of 15 females mated with 5 vasectomized males. Cases were sacrificed and assessed after 36 hr and the endometrial tissue was extracted and kept at -80°C until the next use. The expression of the endometrial receptivity pathway genes, including
RESULTS
RESULTS
The mRNA levels of
CONCLUSION
CONCLUSIONS
Finally, seminal plasma was observed to be effective in expressing the involved genes in the successful implantation pathway, including
Identifiants
pubmed: 33134794
doi: 10.18502/ijrm.v13i10.7765
pmc: PMC7569714
doi:
Types de publication
Journal Article
Langues
eng
Pagination
815-824Informations de copyright
Copyright © 2020 Ajdary et al.
Déclaration de conflit d'intérêts
The authors declare that there is no competing interest.
Références
Immunol Rev. 2005 Aug;206:306-35
pubmed: 16048557
J Endocrinol. 2018 May;237(2):123-137
pubmed: 29535146
Mol Hum Reprod. 2010 Mar;16(3):178-87
pubmed: 19933690
Cell Tissue Res. 2005 Oct;322(1):43-52
pubmed: 15909166
Purinergic Signal. 2014 Mar;10(1):157-87
pubmed: 24271059
J Exp Zool B Mol Dev Evol. 2018 Jun;330(4):215-224
pubmed: 29781132
Immune Netw. 2015 Feb;15(1):16-26
pubmed: 25713505
J Exp Zool. 2002 Feb 1;292(2):129-44
pubmed: 11754029
Exp Ther Med. 2018 Feb;15(2):2109-2119
pubmed: 29434813
Hum Reprod Update. 1999 Jul-Aug;5(4):365-72
pubmed: 10465526
Gynecol Endocrinol. 2018 Feb;34(2):148-152
pubmed: 28868943
Biol Reprod. 2019 Mar 1;100(3):660-671
pubmed: 30329018
Endocrinology. 2017 Jun 1;158(6):1916-1928
pubmed: 28368537
Anim Reprod Sci. 2015 Oct;161:1-15
pubmed: 26300501
Nat Commun. 2018 Feb 9;9(1):603
pubmed: 29426931
Hum Reprod Update. 2011 Mar-Apr;17(2):242-53
pubmed: 20729534
Daru. 2020 Jun 2;:
pubmed: 32483681
Andrology. 2019 Mar;7(2):228-234
pubmed: 30663256
Hum Reprod. 2015 Apr;30(4):800-11
pubmed: 25740886
BMC Womens Health. 2017 Aug 22;17(1):63
pubmed: 28830391
Gynecol Obstet Invest. 1984;18(4):194-8
pubmed: 6510779
J Reprod Immunol. 2002 Oct-Nov;57(1-2):109-28
pubmed: 12385837
Reprod Fertil Dev. 2018 May;30(5):766-778
pubmed: 29157356
Mol Reprod Dev. 2018 Mar;85(3):215-226
pubmed: 29337420
Reprod Med Biol. 2018 Apr 22;17(4):331-342
pubmed: 30377389
Fertil Steril. 1986 Aug;46(2):252-6
pubmed: 3732531
J Cell Physiol. 2018 Jan;234(1):152-157
pubmed: 30078179
Ann Transl Med. 2020 Feb;8(4):99
pubmed: 32175392
Reprod Fertil Dev. 1999;11(2):123-6
pubmed: 10735556
Int J Mol Sci. 2019 Jan 25;20(3):
pubmed: 30691059
J Matern Fetal Neonatal Med. 2020 Aug 2;:1-15
pubmed: 32744104
Hum Reprod. 2003 Jul;18(7):1461-8
pubmed: 12832373
Animal Model Exp Med. 2018 Apr 19;1(1):14-22
pubmed: 30891542
Theriogenology. 2010 Oct 15;74(7):1207-20
pubmed: 20615530
Mol Hum Reprod. 2003 Dec;9(12):785-91
pubmed: 14614040
Soc Reprod Fertil Suppl. 2006;62:231-45
pubmed: 16866321