Comparing cytomegalovirus diagnostics by cell culture and quantitative nucleic acid testing in broncho-alveolar lavage fluids.
Adult
Aged
Aged, 80 and over
Bronchoalveolar Lavage Fluid
/ virology
Cell Culture Techniques
/ methods
Cytomegalovirus
/ genetics
Cytomegalovirus Infections
/ diagnosis
DNA, Viral
/ genetics
Female
Humans
Male
Middle Aged
Prospective Studies
Retrospective Studies
Viral Load
/ methods
Virology
/ methods
Young Adult
bronchoalveolar lavage
cytomegalovirus
nucleic acid testing
pneumonia
virus culture
virus isolation
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
revised:
23
10
2020
received:
16
09
2020
accepted:
30
10
2020
pubmed:
3
11
2020
medline:
9
10
2021
entrez:
2
11
2020
Statut:
ppublish
Résumé
Many clinical laboratories have replaced virus isolation in cell-culture (VIC) for cytomegalovirus (CMV) by quantitative-nucleic-acid testing (QNAT), rendering clinically relevant CMV-replication difficult to distinguish from CMV-shedding or latent infection. We compared direct VIC in 1109 consecutive bronchoalveolar lavage fluids (BALFs) and a well-validated CMV-QNAT (Basel-CMV-UL111a-77bp). In the retrospective Group 1 (N = 694) and Group 2 (N = 303), CMV-QNAT was performed within 48 h from 2-fold and 10-fold concentrated total nucleic acid (TNA) eluates, respectively. In Group 3 (N = 112), 2-fold and 10-fold concentrated TNA eluates were prospectively analyzed in parallel to VIC. CMV was detected by VIC in 79 of 694 (11%) and 26 of 303 (9%) of Groups 1 and 2, but in 114 of 694 (16%) and 57 of 303 (17%) by CMV-QNAT, respectively. Median CMV loads were significantly higher in VIC-positive than in VIC-negative BALF. The likelihood for CMV detection by VIC was 85% for BALF CMV- loads >4 log
Substances chimiques
DNA, Viral
0
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3804-3812Informations de copyright
© 2020 Wiley Periodicals LLC.
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