"FastCheck
Animals
Diagnosis, Differential
Genome, Viral
Goat Diseases
/ diagnosis
Goats
Molecular Diagnostic Techniques
/ methods
Nucleic Acid Amplification Techniques
/ methods
Peste-des-Petits-Ruminants
/ diagnosis
Peste-des-petits-ruminants virus
/ genetics
RNA, Viral
/ isolation & purification
Sensitivity and Specificity
Sheep
Sheep Diseases
/ diagnosis
Small ruminant morbilli virus
differential diagnosis
fast extraction
high-speed RT-qPCR
molecular pen-side test
peste des petits ruminants virus (PPRV)
rapid detection method
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
29 10 2020
29 10 2020
Historique:
received:
03
09
2020
revised:
16
10
2020
accepted:
28
10
2020
entrez:
3
11
2020
pubmed:
4
11
2020
medline:
6
3
2021
Statut:
epublish
Résumé
To assist the global eradication of peste des petits ruminants virus (PPRV), a molecular test for the rapid and reliable detection of PPRV was developed which additionally enables the detection of pathogens relevant for differential diagnostics. For this purpose, the necessary time frame of a magnetic bead-based nucleic acid extraction protocol was markedly shortened to 7 min and 13 s. The optimized extraction was run on a BioSprint 15 platform. Furthermore, a high-speed multi-well RT-qPCR for the genome detection of PPRV and additional important pathogens such as Foot-and-mouth disease virus, Parapoxvirus ovis, Goatpox virus, and
Identifiants
pubmed: 33138260
pii: v12111227
doi: 10.3390/v12111227
pmc: PMC7694148
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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