A Transgenic Line That Reports CSF1R Protein Expression Provides a Definitive Marker for the Mouse Mononuclear Phagocyte System.
Animals
Biomarkers
/ metabolism
Cell Differentiation
/ physiology
Dendritic Cells
/ metabolism
Hematopoietic Stem Cells
/ metabolism
Macrophage Colony-Stimulating Factor
/ metabolism
Macrophages
/ metabolism
Mice
Mice, Inbred C57BL
Mice, Transgenic
Monocytes
/ metabolism
Mononuclear Phagocyte System
/ metabolism
Muscle, Skeletal
/ metabolism
RNA, Messenger
/ metabolism
Receptor, Macrophage Colony-Stimulating Factor
/ metabolism
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
/ metabolism
Tendons
/ metabolism
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
01 12 2020
01 12 2020
Historique:
received:
20
07
2020
accepted:
23
09
2020
pubmed:
4
11
2020
medline:
14
4
2021
entrez:
3
11
2020
Statut:
ppublish
Résumé
The proliferation, differentiation, and survival of cells of the mononuclear phagocyte system (MPS; progenitors, monocytes, macrophages, and classical dendritic cells) are controlled by signals from the M-CSF receptor (CSF1R). Cells of the MPS lineage have been identified using numerous surface markers and transgenic reporters, but none is both universal and lineage restricted. In this article, we report the development and characterization of a CSF1R reporter mouse. A FusionRed (FRed) cassette was inserted in-frame with the C terminus of CSF1R, separated by a T2A-cleavable linker. The insertion had no effect of CSF1R expression or function. CSF1R-FRed was expressed in monocytes and macrophages and absent from granulocytes and lymphocytes. In bone marrow, CSF1R-FRed was absent in lineage-negative hematopoietic stem cells, arguing against a direct role for CSF1R in myeloid lineage commitment. It was highly expressed in marrow monocytes and common myeloid progenitors but significantly lower in granulocyte-macrophage progenitors. In sections of bone marrow, CSF1R-FRed was also detected in osteoclasts, CD169
Identifiants
pubmed: 33139489
pii: jimmunol.2000835
doi: 10.4049/jimmunol.2000835
doi:
Substances chimiques
Biomarkers
0
Csf1r protein, mouse
0
RNA, Messenger
0
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
0
Macrophage Colony-Stimulating Factor
81627-83-0
Receptor, Macrophage Colony-Stimulating Factor
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3154-3166Subventions
Organisme : Medical Research Council
ID : MR/M010341/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M019969/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P013732/1
Pays : United Kingdom
Informations de copyright
Copyright © 2020 by The American Association of Immunologists, Inc.