Initiation of Acupoint Molecular Mechanisms for Manual Acupuncture Analgesia-Nuclear Factor κB Signaling Pathway.
acupoint
analgesia
manual acupuncture
nuclear factor kappa B
Journal
Chinese journal of integrative medicine
ISSN: 1993-0402
Titre abrégé: Chin J Integr Med
Pays: China
ID NLM: 101181180
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
accepted:
01
06
2020
pubmed:
4
11
2020
medline:
15
12
2021
entrez:
3
11
2020
Statut:
ppublish
Résumé
To identify the prominent molecular signaling in acupoints and explore their roles in initiating the analgesia effect of manual acupuncture (MA). A three-step study was conducted, the experiment 1 was a genome-wide analysis of the tissue at acupoint Zusanli (ST 36), including 12 Wistar rats which were divided into control, control+MA1, and control+MA7 groups. In the experiment 2, the paw withdrawal latency (PWL), immunohistochemistry and Western blot analysis of phospho-nuclear factor kappa B (NFκB) p65 (p-p65), phospho-NFκB p50 (p-p50) at ST 36 were performed on rats of saline, saline+MA, and complete Freund's adjuvant (CFA)+MA groups (n=6). In experiment 3, 24 rats were divided into saline+DMSO, CFA+DMSO, CFA+DMSO+MA, and CFA+BAY 11-7082+MA groups, the PWL and immunofluorescence assay of NFκB p65 at ST 36 was conducted. (1) The gene: inhibitor of NFκB (Nfkbia), interleukin-1β (Il1b), interleukin-6 (Il6), chemokine c-x-c motif ligand 1 (Cxcl1), monocyte chemoattractant protein-1 (MCP-1/Ccl2) expressions in the control+MA7 group were significantly increased (P<0.05 or P<0.01), and the expression of NFκB p65 (Rela), NFκB p50 (Nfkb1) were increased in the control+MA7 group (P<0.05). (2) CFA+MA groups showed increased PWL from day 1 to 7 (P<0.01 vs. CFA), and the Western blot results were consistent with immunohistochemistry, the expression of NFκB p-p65 and NFκB p-p50 were significantly increased in the MA-related groups compared with control and CFA groups (P<0.05). (3) Compared with the CFA+DMSO+MA group, the PWL of the CFA+ BAY 11-7082+MA group decreased significantly and continued until day 5 and 7 (P<0.05 and P<0.01, respectively), and the NFκB p65 expression of CFA+BAY 11-7082+MA was significantly reduced compared with CFA+DMSO+MA (P<0.01). Local NFκB signaling cascade in acupoint caused by MA is an important step in initiating the analgesic effect, which would provide new evidence for the initiation of MA-effect and improve the understanding of the scientific basis of acupuncture analgesia.
Identifiants
pubmed: 33140205
doi: 10.1007/s11655-020-3435-6
pii: 10.1007/s11655-020-3435-6
doi:
Substances chimiques
NF-kappa B
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
933-939Informations de copyright
© 2020. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Ernst E. Acupuncture. Lancet Oncol 2010;11:20.
doi: 10.1016/S1470-2045(09)70399-7
Wu JN. A short history of acupuncture. J Alternat Complem Med 1996;2:19–21.
doi: 10.1089/acm.1996.2.19
Vas J, Aranda JM, Modesto M, Benítez PN, Herrera, A, Martínez B, et al. Acupuncture in patients with acute low back pain: a multicentre randomised controlled clinical trial. Pain 2012;153:1883–1889.
doi: 10.1016/j.pain.2012.05.033
Zachary SS, Danesh H. Acupuncture in the management of chronic pain. Topics Pain Manag 2017;32:1–9.
doi: 10.1097/01.TPM.0000516188.16938.92
Chessman AW. Review: acupuncture reduces migraine frequency more than usual care, sham acupuncture, or prophylactic drugs. Ann Intern Med 2016;65:C44.
doi: 10.7326/ACPJC-2016-165-8-044
Greenlee H, Crew KD, Capodice J, Awad D, Jeffres A, Unger JM, et al. Methods to standardize a multicenter acupuncture trial protocol to reduce aromatase inhibitor-related joint symptoms in breast cancer patients. J Acupunct Meridian Stud 2015;8:152–158.
doi: 10.1016/j.jams.2015.03.006
Marchant J. Acupuncture in cancer study reignites debate about controversial technique. Nature 2017;552:157–158.
doi: 10.1038/d41586-017-08309-y
Miller KR, Patel JN, Symanowski JT, Edelen CA, Walsh D. Acupuncture for cancer pain and symptom management in a palliative medicine clinic. Am J Hosp Palliat Care 2019;36:326–332.
doi: 10.1177/1049909118804464
World Health Organization. Acupuncture: review and analysis reports on controlled clinical trials. Geneva 2002;1–81.
World Health Organization. WHO traditional medicine strategy: 2014–2023. Geneva; 2013:1–76.
Cressey D. Acupuncture for mice. Nature 2010;465:538.
doi: 10.1038/465538a
Fan GQ, Qian LL, Zhao Y, Fu ZH. Acupuncture analgesia:variousness and analysis. Chin Acupunct Moxibust (Chin) 2013;33:92–96.
Zhang K, Ding SS, Gong YN, Zhao X, Kai Li. Initiation mechanisms of acupuncture effect: a literature review of basic studies. J Acupunct Tuina Sci (Chin) 2020;18:75–82.
doi: 10.1007/s11726-019-1149-0
Huang M, Wang XZ, Xing BB, Yang HW, Sa ZY, Zhang D, et al. Critical roles of TRPV2 channels, histamine H1 and adenosine A1 receptors in the initiation of acupoint signals for acupuncture analgesia. Sci Rep 2018;8:6523.
doi: 10.1038/s41598-018-24654-y
Park JY, Park JJ, Jeon S, Doo AR, Kim SN, Lee HS, et al. From peripheral to central: the role of ERK signaling pathway in acupuncture analgesia. J Pain 2014;15:535–549.
doi: 10.1016/j.jpain.2014.01.498
Qi J, Chen C, Meng QX, Wu Y, Wu H, Zhao TB. Crosstalk between activated microglia and neurons in the spinal dorsal horn contributes to stress-induced hyperalgesia. Sci Rep 2016;6:39442.
doi: 10.1038/srep39442
Chen CY, Lin CN, Chern RS, Tsai YC, Chang YH, Chien CH. Neuronal activity stimulated by liquid substrates injection at Zusanli (ST36) acupoint: the possible mechanism of aquapuncture. Evid Based Complement Alternat Med 2014;2014:627342.
pubmed: 24734109
pmcid: 3963215
Goldman N, Chen M, Fujita T, Xu Q, Peng W, Liu W, et al. Adenosine A1 receptors mediate local anti-nociceptive effects of acupuncture. Nature Neurosci 2010;53:38–39.
Cui X, Liu K, Xu D, Zhang Y, He X, Liu H, et al. Mast cell deficiency attenuates acupuncture analgesia for mechanical pain using c-kit gene mutant rats. J Pain Res 2018;11:483–495.
doi: 10.2147/JPR.S152015
Zhou W, Lei RX, Zuo CY, Yue YQ, Luo Q, Zhang CS, et al. Analgesic effect of moxibustion with different temperature on inflammatory and neuropathic pain mice: a comparative study. Evid Based Complement Alternat Med 2017;2017:4373182.
pubmed: 29234396
pmcid: 5654268
Xu Y, Hong SH, Zhao X, Wang SJ, Xu ZF, Ding SS, et al. Acupuncture alleviates rheumatoid arthritis by immune-network modulation. Am J Chin Med 2018;46:997–1019.
doi: 10.1142/S0192415X18500520
Zhu D, Fan T, Huo X, Cui J, Cheung CW, Xia Z. Progressive increase of inflammatory CXCR4 and TNF-alpha in the dorsal root ganglia and spinal cord maintains peripheral and central sensitization to diabetic neuropathic pain in rats. Mediators Inflamm 2019;2019:4856156.
pubmed: 31001066
pmcid: 6437743
Zhang K, Guo XM, Yan YW, Liu YY, Xu ZF, Zhao X, et al. Applying statistical and complex network methods to explore the key signaling molecules of acupuncture regulating neuroendocrine-immune network. Evid Based Complement Alternat Med 2018;2018:9260630.
pubmed: 29785197
pmcid: 5896411
Oeckinghaus A, Hayden MS, Ghosh S. Crosstalk in NF-κ B signaling pathways. Nat Immunol 2011;12:695–708.
doi: 10.1038/ni.2065
Olga K, Volodymyr K, Angela S, Andrij S, Viacheslav VK, Victor D, et al. Spinal PKCα inhibition and gene-silencing for pain relief: AMPAR trafficking at the synapses between primary afferents and sensory interneurons. Sci Rep 2018;8:10285.
doi: 10.1038/s41598-018-28512-9
Pinho-Ribeiro FA, Verri WA Jr, Chiu IM. Nociceptor sensory neuron-immune interactions in pain and inflammation. Trends Immunol 2017;38:5–19.
doi: 10.1016/j.it.2016.10.001
Li NC, Li MY, Chen B, Guo Y. A new perspective of acupuncture: the interaction among three networks leads to neutralization. Evid Based Complement Alternat Med 2019;2019:2326867.
pubmed: 30915143
pmcid: 6409053