The Nontypeable Haemophilus influenzae Major Adhesin Hia Is a Dual-Function Lectin That Binds to Human-Specific Respiratory Tract Sialic Acid Glycan Receptors.
Adhesins, Bacterial
/ chemistry
Amino Acid Sequence
Binding Sites
Biofilms
Haemophilus Infections
/ metabolism
Haemophilus influenzae
/ physiology
Host-Pathogen Interactions
Humans
Models, Molecular
Molecular Conformation
Molecular Structure
Protein Binding
Receptors, Cell Surface
/ metabolism
Respiratory Mucosa
/ metabolism
COPD
NTHi
adhesin
autotransporter proteins
bacterial pathogen
glycan
host cell
middle ear infection
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
03 11 2020
03 11 2020
Historique:
entrez:
4
11
2020
pubmed:
5
11
2020
medline:
8
9
2021
Statut:
epublish
Résumé
NTHi is a human-adapted pathogen that colonizes the human respiratory tract. Strains of NTHi express multiple adhesins; however, there is a unique, mutually exclusive relationship between the major adhesins Hia and HMW1 and HMW2 (HMW1/2). Approximately 25% of NTHi strains express Hia, a phase-variable autotransporter protein that has a critical role in colonization of the host nasopharynx. The remaining 75% of strains express HMW1/2. Previous work has shown that the HMW1 and HMW2 proteins mediate binding to 2-3- and 2-6-linked sialic acid glycans found in the human respiratory tract. Here, we show that the high-affinity binding domain of Hia, binding domain 1 (BD1), is responsible for binding to α2-6-sialyllactosamine (2-6 SLN) glycans. BD1 is highly specific for glycans that incorporate the form of sialic acid expressed by humans,
Identifiants
pubmed: 33144377
pii: mBio.02714-20
doi: 10.1128/mBio.02714-20
pmc: PMC7642680
pii:
doi:
Substances chimiques
Adhesins, Bacterial
0
Hia adhesin
0
Receptors, Cell Surface
0
sialic acid receptor
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIDCD NIH HHS
ID : R01 DC015688
Pays : United States
Informations de copyright
Copyright © 2020 Atack et al.
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