Jumping the green wall: The use of PNA-DNA clamps to enhance microbiome sampling depth in wildlife microbiome research.
16s
PCR blockers
PNAs
chloroplast
mitochondria
wildlife microbiome
Journal
Ecology and evolution
ISSN: 2045-7758
Titre abrégé: Ecol Evol
Pays: England
ID NLM: 101566408
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
19
06
2020
revised:
12
08
2020
accepted:
24
08
2020
entrez:
4
11
2020
pubmed:
5
11
2020
medline:
5
11
2020
Statut:
epublish
Résumé
As microbiome research moves away from model organisms to wildlife, new challenges for microbiome high-throughput sequencing arise caused by the variety of wildlife diets. High levels of contamination are commonly observed emanating from the host (mitochondria) or diet (chloroplast). Such high contamination levels affect the overall sequencing depth of wildlife samples thus decreasing statistical power and leading to poor performance in downstream analysis. We developed an amplification protocol utilizing PNA-DNA clamps to maximize the use of resources and to increase the sampling depth of true microbiome sequences in samples with high levels of plastid contamination. We chose two study organisms, a bat (
Identifiants
pubmed: 33145000
doi: 10.1002/ece3.6814
pii: ECE36814
pmc: PMC7593133
doi:
Banques de données
Dryad
['10.5061/dryad.4tmpg4f7j']
Types de publication
Journal Article
Langues
eng
Pagination
11779-11786Informations de copyright
© 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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