Microbiota of semen from stallions in Sweden identified by MALDI-TOF.
Contaminants
Equine semen
MALDI-TOF
Microorganism
Non-pathogenic bacteria
Semen extender
Journal
Veterinary and animal science
ISSN: 2451-943X
Titre abrégé: Vet Anim Sci
Pays: Netherlands
ID NLM: 101694897
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
28
04
2020
revised:
25
09
2020
accepted:
25
09
2020
entrez:
4
11
2020
pubmed:
5
11
2020
medline:
5
11
2020
Statut:
epublish
Résumé
Stallion semen is known to contain environmental bacteria and normal commensals, and in some cases may contain opportunistic pathogens. These bacteria may negatively influence sperm quality during storage before artificial insemination. The bacteria isolated depend on the culture conditions and method of identification; therefore, the aim of this study was to identify as many of the bacteria present in stallion semen as possible by culturing aliquots of semen under a variety of conditions. Eleven semen samples were available: five extended semen samples from one stud together with a sample of the extender, and six raw semen samples from another stud. Aliquots of semen samples were cultured on different agars and under specialized conditions; individual bacterial colonies were identified using Matrix-assisted laser desorption ionization time of flight mass spectrometry. Approximately 55% of the bacteria could be identified, with 20 bacterial taxa being isolated from semen samples from the five stallions on the first stud and 11 taxa from the semen samples from six stallions on the second stud.
Identifiants
pubmed: 33145452
doi: 10.1016/j.vas.2020.100143
pii: S2451-943X(20)30056-9
pmc: PMC7593612
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100143Informations de copyright
© 2020 The Author(s).
Déclaration de conflit d'intérêts
The authors do not have any conflict of interest to declare.
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