3D virtual histology of human pancreatic tissue by multiscale phase-contrast X-ray tomography.
3D histology
X-ray tomography
biopsy
pancreas
phase retrieval
Journal
Journal of synchrotron radiation
ISSN: 1600-5775
Titre abrégé: J Synchrotron Radiat
Pays: United States
ID NLM: 9888878
Informations de publication
Date de publication:
01 Nov 2020
01 Nov 2020
Historique:
received:
06
07
2020
accepted:
18
08
2020
entrez:
4
11
2020
pubmed:
5
11
2020
medline:
20
8
2021
Statut:
ppublish
Résumé
A multiscale three-dimensional (3D) virtual histology approach is presented, based on two configurations of propagation phase-contrast X-ray tomography, which have been implemented in close proximity at the GINIX endstation at the beamline P10/PETRA III (DESY, Hamburg, Germany). This enables the 3D reconstruction of characteristic morphological features of human pancreatic normal and tumor tissue, as obtained from cancer surgery, first in the form of a large-scale overview by parallel-beam illumination, followed by a zoom into a region-of-interest based on zoom tomography using a Kirkpatrick-Baez mirror with additional waveguide optics. To this end 1 mm punch biopsies of the tissue were taken. In the parallel tomography, a volumetric throughput on the order of 0.01 mm
Identifiants
pubmed: 33147198
pii: S1600577520011327
doi: 10.1107/S1600577520011327
pmc: PMC7642968
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1707-1719Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : 05K19MG2
Organisme : Deutsche Forschungsgemeinschaft
ID : EXC2067
Informations de copyright
open access.
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