Epinephrine penetrates through gingival sulcus unlike keratinized gingiva and evokes remote vasoconstriction in human.
Blood flow
Epinephrine
Gingiva
Spreading vasoconstriction
Journal
BMC oral health
ISSN: 1472-6831
Titre abrégé: BMC Oral Health
Pays: England
ID NLM: 101088684
Informations de publication
Date de publication:
04 11 2020
04 11 2020
Historique:
received:
14
07
2020
accepted:
22
10
2020
entrez:
5
11
2020
pubmed:
6
11
2020
medline:
30
12
2020
Statut:
epublish
Résumé
It has been demonstrated in non-oral tissues that the locally evoked vasoconstriction could elicit remote vasoconstriction. This study aimed to investigate the spreading vasoconstrictor effects of epinephrine in the gingiva. Gingival blood flow (GBF) was measured by laser speckle contrast imager in 21 healthy volunteers. In group A, two wells were fabricated from orthodontic elastic ligature and placed 2 mm apically to the free gingival margin at the mid buccal line of 12 (test side) and 21 (control side) teeth. The GBF was measured in the wells and tightly apical, coronal, distal and mesial to the wells. In group B, the wells were made on the buccal surface of the same teeth, including the gingival sulcus. Four regions were selected for measurement from the gingival margin reaching the mucogingival line (coronal, midway1, midway2 and apical). After the baseline recording, 3 µg epinephrine was applied into the test, and physiological saline into the control well. The GBF was recorded for 14 min. The gingival thickness was measured with a PIROP Ultrasonic Biometer. In group A, the GBF did not increase or decrease after the application of epinephrine. In group B, the GBF significantly decreased in all regions of the test side and remained low for the observation period. The vasoconstriction appeared with delays in more apical regions (at min 1 in the coronal and the midway1, at min 2 in the midway2, at min 4 in the apical region). Similarly, the amount of the decrease at 14 min was the largest close to sulcus (- 53 ± 2.9%), followed by the midway1 (- 51 ± 2.8%) and midway2 (- 42 ± 4.2%) and was the lowest in the apical region (- 32 ± 5.8%). No correlation was found between GBF and gingival thickness. Epinephrine could evoke intense vasoconstriction propagating to the mucogingival junction, indicating the presence of spreading vasoconstriction in the human gingiva. The attached gingiva is impermeable to epinephrine, unlike the gingival sulcus. This trial was registered in ClinicalTrials.gov titled as Evidence of Spreading Vasoconstriction in Human Gingiva with the reference number of NCT04131283 on 16 October 2019. https://clinicaltrials.gov/show/NCT04131283.
Sections du résumé
BACKGROUND
It has been demonstrated in non-oral tissues that the locally evoked vasoconstriction could elicit remote vasoconstriction. This study aimed to investigate the spreading vasoconstrictor effects of epinephrine in the gingiva.
METHODS
Gingival blood flow (GBF) was measured by laser speckle contrast imager in 21 healthy volunteers. In group A, two wells were fabricated from orthodontic elastic ligature and placed 2 mm apically to the free gingival margin at the mid buccal line of 12 (test side) and 21 (control side) teeth. The GBF was measured in the wells and tightly apical, coronal, distal and mesial to the wells. In group B, the wells were made on the buccal surface of the same teeth, including the gingival sulcus. Four regions were selected for measurement from the gingival margin reaching the mucogingival line (coronal, midway1, midway2 and apical). After the baseline recording, 3 µg epinephrine was applied into the test, and physiological saline into the control well. The GBF was recorded for 14 min. The gingival thickness was measured with a PIROP Ultrasonic Biometer.
RESULTS
In group A, the GBF did not increase or decrease after the application of epinephrine. In group B, the GBF significantly decreased in all regions of the test side and remained low for the observation period. The vasoconstriction appeared with delays in more apical regions (at min 1 in the coronal and the midway1, at min 2 in the midway2, at min 4 in the apical region). Similarly, the amount of the decrease at 14 min was the largest close to sulcus (- 53 ± 2.9%), followed by the midway1 (- 51 ± 2.8%) and midway2 (- 42 ± 4.2%) and was the lowest in the apical region (- 32 ± 5.8%). No correlation was found between GBF and gingival thickness.
CONCLUSION
Epinephrine could evoke intense vasoconstriction propagating to the mucogingival junction, indicating the presence of spreading vasoconstriction in the human gingiva. The attached gingiva is impermeable to epinephrine, unlike the gingival sulcus. This trial was registered in ClinicalTrials.gov titled as Evidence of Spreading Vasoconstriction in Human Gingiva with the reference number of NCT04131283 on 16 October 2019. https://clinicaltrials.gov/show/NCT04131283.
Identifiants
pubmed: 33148235
doi: 10.1186/s12903-020-01296-z
pii: 10.1186/s12903-020-01296-z
pmc: PMC7640651
doi:
Substances chimiques
Vasoconstrictor Agents
0
Epinephrine
YKH834O4BH
Banques de données
ClinicalTrials.gov
['NCT04131283']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
305Subventions
Organisme : Hungarian Scientific Research Fund
ID : K112364
Pays : International
Organisme : Hungarian Human Resource Development Operational Programme
ID : EFOP-3.6.2-16-2017-00006
Pays : International
Références
Am J Physiol. 1989 Mar;256(3 Pt 2):H832-7
pubmed: 2923241
J Clin Periodontol. 1991 Mar;18(3):204-9
pubmed: 2061421
Int J Oral Surg. 1980 Apr;9(2):144-7
pubmed: 6773898
Crit Rev Oral Biol Med. 1991;2(1):13-32
pubmed: 1912142
Arch Oral Biol. 1967 May;12(5):611-21
pubmed: 5336946
J Clin Periodontol. 2007 May;34(5):437-43
pubmed: 17448047
J Clin Periodontol. 1979 Dec;6(6):417-24
pubmed: 295289
Gen Dent. 2000 May-Jun;48(3):299-302
pubmed: 11199596
Neurosci Lett. 1990 Apr 20;112(1):65-9
pubmed: 2385366
J Dent Anesth Pain Med. 2018 Jun;18(3):143-149
pubmed: 29984318
Biomed Res Int. 2017;2017:4042902
pubmed: 28232940
J Oral Surg (Chic). 1955 Oct;13(4):299-306
pubmed: 13264006
Auton Neurosci. 2015 Jul;190:10-9
pubmed: 25854799
Expert Rev Cardiovasc Ther. 2014 May;12(5):557-64
pubmed: 24745959
J Prosthet Dent. 2007 Jan;97(1):6-11
pubmed: 17280885
Anesthesiology. 1989 Nov;71(5):757-62
pubmed: 2817471
Microcirculation. 2018 Apr;25(3):e12446
pubmed: 29457306
Int J Periodontics Restorative Dent. 2019 Mar/Apr;39(2):e64-e70
pubmed: 30794263
Acta Odontol Scand. 1998 Aug;56(4):220-8
pubmed: 9765014
Br J Oral Maxillofac Surg. 2013 Dec;51(8):928-31
pubmed: 23958351
PLoS One. 2015 Aug 13;10(8):e0133760
pubmed: 26270037
Br J Pharmacol. 1979 Oct;67(2):207-15
pubmed: 40647
Microvasc Res. 1983 Jul;26(1):27-35
pubmed: 6888285
Clin Oral Implants Res. 2007 Feb;18(1):133-9
pubmed: 17224034
Circ Res. 1970 Feb;26(2):163-70
pubmed: 5412532
Arch Oral Biol. 2010 May;55(5):343-9
pubmed: 20378098
Pharmacol Ther. 1999 Feb;81(2):141-61
pubmed: 10190584
J Periodontol. 1981 Jan;52(1):1-7
pubmed: 6162939
Oper Dent. 2002 Jul-Aug;27(4):343-8
pubmed: 12120770
J Athl Train. 2011 May-Jun;46(3):277-81
pubmed: 21669097
Microvasc Res. 2012 Mar;83(2):249-56
pubmed: 22080047
Int J Oral Surg. 1979 Aug;8(4):301-6
pubmed: 120334
Vojnosanit Pregl. 2014 Jan;71(1):46-51
pubmed: 24516990
Oral Dis. 2019 Oct;25(7):1780-1788
pubmed: 31336001
J Esthet Restor Dent. 2019 May;31(3):263-267
pubmed: 30520211
Ann Phys Med. 1954 Apr;2(2):44-7
pubmed: 13208094
Oral Surg Oral Med Oral Pathol. 1981 Dec;52(6):577-82
pubmed: 6947180
Eur Heart J. 2011 Apr;32(7):784-7
pubmed: 21169282
Clin Hemorheol Microcirc. 2016;64(3):255-266
pubmed: 27858702
Microcirculation. 2018 Feb;25(2):
pubmed: 28976050
Exp Ther Med. 2020 Mar;19(3):2037-2044
pubmed: 32104263
J Plast Reconstr Aesthet Surg. 2017 Mar;70(3):322-329
pubmed: 27939906
J Oral Implantol. 2018 Oct;44(5):378-383
pubmed: 29870305
J Biophotonics. 2018 Dec;11(12):e201800242
pubmed: 30112807
Anesthesiology. 1991 Aug;75(2):243-50
pubmed: 1859013
Am J Physiol. 1998 Jan;274(1):H178-86
pubmed: 9458866
J Physiol. 1999 Apr 1;516 ( Pt 1):283-91
pubmed: 10066941
Anesth Prog. 2016 Spring;63(1):17-24
pubmed: 26866407
J Biomed Opt. 2014 Aug;19(8):086001
pubmed: 25089945
J Prosthet Dent. 1985 Apr;53(4):525-31
pubmed: 3889287
Pharmacol Rev. 1980 Dec;32(4):337-62
pubmed: 6267618
J Clin Periodontol. 1997 Dec;24(12):888-92
pubmed: 9442425
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998 Feb;85(2):197-202
pubmed: 9503456
Front Immunol. 2019 Feb 14;10:208
pubmed: 30837987
J Periodontol. 1969 Mar;40(3):137-41
pubmed: 5253597
J Periodontal Res. 1989 Jan;24(1):45-52
pubmed: 2524569
J Periodontal Res. 2019 Oct;54(5):499-505
pubmed: 30865289
J Vis Exp. 2019 Jan 12;(143):
pubmed: 30688301
Anesth Prog. 1999 Spring;46(2):71-6
pubmed: 10853568
Am J Physiol. 1995 Dec;269(6 Pt 2):H2022-30
pubmed: 8594912