Evolution from adherent to suspension: systems biology of HEK293 cell line development.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 11 2020
04 11 2020
Historique:
received:
11
02
2020
accepted:
22
10
2020
entrez:
5
11
2020
pubmed:
6
11
2020
medline:
11
3
2021
Statut:
epublish
Résumé
The need for new safe and efficacious therapies has led to an increased focus on biologics produced in mammalian cells. The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of several therapeutic proteins and viral vectors. Despite the increased popularity of this strain we still have limited knowledge on the genetic composition of its derivatives. Here we present a genomic, transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines. Changes in gene copy and expression between industrial progeny cell lines and the original HEK293 were associated with cellular component organization, cell motility and cell adhesion. Changes in gene expression between adherent and suspension derivatives highlighted switching in cholesterol biosynthesis and expression of five key genes (RARG, ID1, ZIC1, LOX and DHRS3), a pattern validated in 63 human adherent or suspension cell lines of other origin.
Identifiants
pubmed: 33149219
doi: 10.1038/s41598-020-76137-8
pii: 10.1038/s41598-020-76137-8
pmc: PMC7642379
doi:
Substances chimiques
Cholesterol
97C5T2UQ7J
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18996Commentaires et corrections
Type : ErratumIn
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