Development of a swine RNA polymerase I driven Influenza reverse genetics system for the rescue of type A and B Influenza viruses.
Human cells
Influenza virus
Reverse genetics
Swine RNA polymerase I
Swine cells
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
14
04
2020
revised:
26
10
2020
accepted:
30
10
2020
pubmed:
6
11
2020
medline:
25
11
2021
entrez:
5
11
2020
Statut:
ppublish
Résumé
Influenza viruses are among the most significant pathogens of humans and animals. Reverse genetics allows for the study of molecular attributes that modulate virus host range, virulence and transmission. The most common reverse genetics methods use bi-directional vectors containing a host RNA polymerase (pol) I promoter to produce virus-like RNAs and a host RNA pol II promoter to direct the synthesis of viral proteins. Given the species-dependency of the pol I promoter and virus-host interactions that influence replication of animal-origin influenza viruses in human-derived cells, we explored the potential of using the swine RNA pol I promoter (spol1) in a bi-directional vector for rescuing type A and B influenza viruses (IAV and IBV, respectively) in swine and human cells. The spol1-based bi-directional plasmid vector led to efficient rescue of IAVs of different origins (human, swine, and avian) as well as IBV in both swine- and human-origin tissue culture cells. In addition, virus rescue was successful using a recombinant bacmid containing all eight segments of a swine origin IAV. In conclusion, the spol1-based reverse genetics system is a new platform to study influenza viruses and produce swine influenza vaccines with increased transfection efficiency.
Identifiants
pubmed: 33152409
pii: S0166-0934(20)30263-9
doi: 10.1016/j.jviromet.2020.114011
pmc: PMC8103788
mid: NIHMS1697645
pii:
doi:
Substances chimiques
Influenza Vaccines
0
RNA Polymerase I
EC 2.7.7.6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
114011Subventions
Organisme : NIAID NIH HHS
ID : HHSN272201400008C
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI146448
Pays : United States
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
Références
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
Virus Genes. 2017 Aug;53(4):661-666
pubmed: 28434065
J Virol. 2014 Sep 1;88(17):10013-25
pubmed: 24942589
PLoS One. 2012;7(9):e46378
pubmed: 23029501
Vet Microbiol. 2017 Jul;206:35-44
pubmed: 27923501
PLoS Pathog. 2010 Oct 14;6(10):e1001145
pubmed: 20976194
J Virol. 2014 Sep;88(18):10778-91
pubmed: 25008914
J Virol. 2007 Sep;81(17):9238-48
pubmed: 17596317
Methods Mol Biol. 2020;2123:37-59
pubmed: 32170679
Science. 2009 Jul 24;325(5939):484-7
pubmed: 19574347
Front Microbiol. 2018 Feb 06;9:123
pubmed: 29467737
Nat Commun. 2019 Aug 6;10(1):3526
pubmed: 31387995
J Virol. 2017 May 26;91(12):
pubmed: 28381580
J Virol. 2015 May;89(9):4818-26
pubmed: 25673727
Proc Natl Acad Sci U S A. 2000 May 23;97(11):6108-13
pubmed: 10801978
J Virol. 2013 Jan;87(1):384-94
pubmed: 23077313
J Virol. 2011 Jan;85(1):456-69
pubmed: 20962084
J Gen Virol. 2012 Oct;93(Pt 10):2204-2214
pubmed: 22815274
DNA Cell Biol. 1996 Feb;15(2):167-73
pubmed: 8634144
PLoS One. 2011;6(12):e27989
pubmed: 22163276
Arch Virol. 2001 Dec;146(12):2275-89
pubmed: 11811679
Virology. 2015 Feb;476:206-216
pubmed: 25553516
Curr Opin Genet Dev. 1995 Oct;5(5):652-6
pubmed: 8664554