Vaginal Microbiota Is Stable throughout the Estrous Cycle in Arabian Maress.
diestrus
equine
estrus
horses
metagenomic
microbiome
reproductive
vagina
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
03 Nov 2020
03 Nov 2020
Historique:
received:
16
09
2020
revised:
23
10
2020
accepted:
28
10
2020
entrez:
6
11
2020
pubmed:
7
11
2020
medline:
7
11
2020
Statut:
epublish
Résumé
Lactic acid bacteria (LAB) dominate human vaginal microbiota and inhibit pathogen proliferation. In other mammals, LAB do not dominate vaginal microbiota, however shifts of dominant microorganisms occur during ovarian cycle. The study objectives were to characterize equine vaginal microbiota in mares by culture-dependent and independent methods and to describe its variation in estrus and diestrus. Vaginal swabs from 8 healthy adult Arabian mares were obtained in estrus and diestrus. For culture-dependent processing, bacteria were isolated on Columbia blood agar (BA) and Man Rogosa Sharpe (MRS) agar. LAB comprised only 2% of total bacterial isolates and were not related to ovarian phases. For culture-independent processing, V3/V4 variable regions of the 16S ribosomal RNA gene were amplified and sequenced using Illumina Miseq. The diversity and composition of the vaginal microbiota did not change during the estrous cycle. Core equine vaginal microbiome consisted of Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria at the phylum level. At the genus level it was defined by
Identifiants
pubmed: 33153053
pii: ani10112020
doi: 10.3390/ani10112020
pmc: PMC7692283
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Universidad CEU Cardenal Herrera
ID : PRCEU-UCH 04/11
Organisme : Ramón y Cajal contract of the Spanish Ministry of Science, Innovation and Universities
ID : RYC-2018-024985-I (J.J.Q)
Organisme : Programa FUSP-Santander (to Ángel Gómez)
ID : FUSP-Santander
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