Expression and Localization of Paneth Cells and Their α-Defensins in the Small Intestine of Adult Mouse.
Institute of Cancer Research mouse
Paneth cell
alpha-defensin
cryptdin
germ-free mouse
innate immunity
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
07
06
2020
accepted:
15
09
2020
entrez:
6
11
2020
pubmed:
7
11
2020
medline:
1
6
2021
Statut:
epublish
Résumé
Paneth cells contribute to intestinal innate immunity by sensing bacteria and secreting α-defensin. In Institute of Cancer Research (ICR) mice, α-defensin termed cryptdin (Crp) in Paneth cells consists of six major isoforms, Crp1 to 6. Despite accumulating evidences that α-defensin functions in controlling the intestinal microbiota, topographical localization of Paneth cells in the small intestine in relation to functions of α-defensin remains to be determined. In this study, we examined the expression level of messenger RNA (mRNA) encoding six Crp-isoforms and Crp immunoreactivities using singly isolated crypts together with bactericidal activities of Paneth cell secretions from isolated crypts of duodenum, jejunum, and ileum. Here we showed that levels of Crp mRNAs in the single crypt ranged from 5 x 10
Identifiants
pubmed: 33154750
doi: 10.3389/fimmu.2020.570296
pmc: PMC7590646
doi:
Substances chimiques
Anti-Infective Agents
0
Protein Precursors
0
RNA, Messenger
0
alpha-Defensins
0
cryptdin 4, mouse
0
cryptdin
120668-29-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
570296Informations de copyright
Copyright © 2020 Nakamura, Yokoi, Fukaya, Ohira, Shinozaki, Nishida, Kikuchi and Ayabe.
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