Suppression of MKL1 promotes adipocytic differentiation and reduces the proliferation of myxoid liposarcoma cells.
MKL1
Oil Red O staining
adipocytic differentiation
differentiation therapy
myxoid liposarcoma
Journal
Oncology letters
ISSN: 1792-1074
Titre abrégé: Oncol Lett
Pays: Greece
ID NLM: 101531236
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
31
01
2020
accepted:
23
09
2020
entrez:
6
11
2020
pubmed:
7
11
2020
medline:
7
11
2020
Statut:
ppublish
Résumé
Myxoid liposarcoma (MLS) is thought to occur due to defective adipocytic differentiation in mesenchymal stem cells. A promising strategy for MLS treatment is the prevention of sarcomagenesis by promoting the terminal differentiation of MLS cells into adipocytes. Previous studies have reported that the suppression of megakaryoblastic leukemia 1 (MKL1) expression induces adipocytic differentiation in preadipocyte cell lines. The present study aimed to investigate the effects of MKL1 suppression on MLS cells. In the present study, MKL1 knockdown was demonstrated to promote the adipocytic differentiation of an MLS-derived cell line, designated 1955/91, under adipogenic conditions. This suggests that therapeutic targeting of the MKL1-associated molecular pathway has potential as a promising method of MLS treatment. However, the induction of adipogenesis by MKL knockdown was incomplete, and Oil Red O staining indicated that intracellular lipid droplets were only sporadically generated. Conversely, MKL1 knockdown reduced the growth of the MLS cells. As adipocytic differentiation
Identifiants
pubmed: 33154767
doi: 10.3892/ol.2020.12232
pii: OL-0-0-12232
pmc: PMC7608058
doi:
Types de publication
Journal Article
Langues
eng
Pagination
369Informations de copyright
Copyright © 2020, Spandidos Publications.
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