Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression Program.
Adenocarcinoma
/ genetics
Alternative Splicing
Animals
Cell Cycle Proteins
/ metabolism
Exons
Gene Expression Profiling
/ methods
Genes, Tumor Suppressor
Humans
Liver Neoplasms
/ genetics
Male
Mice
Mice, Inbred ICR
Mice, SCID
RNA Interference
RNA Splicing
RNA-Binding Proteins
/ genetics
Tumor Suppressor Protein p53
/ genetics
CRISPR screen
Mdm4
RBP
RNAi screen
Zmat3
alternative splicing
hepatocellular carcinoma
lung adenocarcinoma
p53
tumor suppression
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
05 11 2020
05 11 2020
Historique:
received:
13
02
2020
revised:
19
08
2020
accepted:
14
10
2020
entrez:
6
11
2020
pubmed:
7
11
2020
medline:
5
1
2021
Statut:
ppublish
Résumé
Although TP53 is the most commonly mutated gene in human cancers, the p53-dependent transcriptional programs mediating tumor suppression remain incompletely understood. Here, to uncover critical components downstream of p53 in tumor suppression, we perform unbiased RNAi and CRISPR-Cas9-based genetic screens in vivo. These screens converge upon the p53-inducible gene Zmat3, encoding an RNA-binding protein, and we demonstrate that ZMAT3 is an important tumor suppressor downstream of p53 in mouse Kras
Identifiants
pubmed: 33157015
pii: S1097-2765(20)30729-2
doi: 10.1016/j.molcel.2020.10.022
pmc: PMC7654708
mid: NIHMS1640952
pii:
doi:
Substances chimiques
Cell Cycle Proteins
0
RNA-Binding Proteins
0
Tumor Suppressor Protein p53
0
ZMAT3 protein, human
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
452-469.e9Subventions
Organisme : NCI NIH HHS
ID : T32 CA009302
Pays : United States
Organisme : NCI NIH HHS
ID : F32 CA162681
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA087497
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA023100
Pays : United States
Organisme : NHGRI NIH HHS
ID : K99 HG009530
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA233944
Pays : United States
Organisme : NCI NIH HHS
ID : R35 CA197591
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests E.L.V.N. is cofounder, member of the Board of Directors, on the scientific advisory board, equity holder, and paid consultant for Eclipse BioInnovations. G.W.Y. is cofounder, member of the Board of Directors, on the scientific advisory board, equity holder, and paid consultant for Locana and Eclipse BioInnovations. G.W.Y. is a visiting professor at the National University of Singapore. Dr. Yeo’s interest(s) have been reviewed and approved by UCSD in accordance with its conflict of interest policies. S.W.L. is a member of the scientific advisory board of PMV Pharmaceuticals. The authors declare no other competing financial interests.
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