The Effect of Naja naja oxiana Snake Venom Against Leishmania tropica Confirmed by Advanced Assays.
Leishmania tropica
Leishmanicidal effect
Mechanism of action
Snake venom fractions
Journal
Acta parasitologica
ISSN: 1896-1851
Titre abrégé: Acta Parasitol
Pays: Switzerland
ID NLM: 9301947
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
15
07
2020
accepted:
13
10
2020
pubmed:
8
11
2020
medline:
31
7
2021
entrez:
7
11
2020
Statut:
ppublish
Résumé
The aim of this study was to explore the activity of Naja naja oxiana venom on Leishmania tropica and its modes of action. Different fractions of Naja naja oxiana venom (NNOV) were prepared and characterized by high-performance liquid chromatography. The superior component, fraction k (FK) was selected. The activity of the fraction was assessed using advanced assays. Interleukin (IL)-12, TNF-α and iNOS gene expression as the indicators of Th1 significantly increased. In contrast, the level of IL-10, as the marker of T helper 2 substantially decreased (p < 0.001). Reactive oxygen species (ROS) detection showed a significant increase (p < 0.001) after treatment with different concentrations of NNOV-FK, unlike arginase (L-ARG) activity which showed a significant reduction (p < 0.001). The NNOV-FK showed significant lethal activity on the L. tropica stages. The findings demonstrated that NNOV-FK represented a strong leishmanicidal activity on L. tropica stages. The major modes of NNOV-FK action are multidimensional, which perceives the induction of a synergistic response and upregulation of the immune-modulatory role towards Th1 response against L. tropica stages as well as apoptotic and anti-metabolic action as a model drug to generate ROS, block the polyamine synthesis and lead to parasite death.
Identifiants
pubmed: 33159262
doi: 10.1007/s11686-020-00301-3
pii: 10.1007/s11686-020-00301-3
doi:
Substances chimiques
Elapid Venoms
0
Snake Venoms
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
475-486Références
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