An alternative cold chain for storing and transporting East Coast fever vaccine.
Cryopreservation
Dry ice
ECF
East Coast Fever
Muguga cocktail vaccine
Theileria parva
Journal
Veterinary parasitology
ISSN: 1873-2550
Titre abrégé: Vet Parasitol
Pays: Netherlands
ID NLM: 7602745
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
18
08
2020
revised:
31
10
2020
accepted:
01
11
2020
pubmed:
9
11
2020
medline:
30
6
2021
entrez:
8
11
2020
Statut:
ppublish
Résumé
East Coast fever (ECF) is an often fatal, economically important cattle disease that predominantly affects eastern, central, and southern Africa. ECF is controlled through vaccination by means of simultaneous injection of oxytetracycline and cryogenically preserved stabilate containing live, disease-causing parasites. Storage and transportation of the stabilate requires liquid nitrogen, a commodity that is commonly unreliable in low-resource settings. Here we show that storage of conventionally prepared stabilate at -80 °C for up to 30 days does not significantly affect its ability to infect cultured peripheral blood mononucleated cells or live cattle, suggesting an alternative cold chain that maintains these temperatures could be used to effectively manage ECF.
Identifiants
pubmed: 33161281
pii: S0304-4017(20)30284-3
doi: 10.1016/j.vetpar.2020.109304
pii:
doi:
Substances chimiques
Protozoan Vaccines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109304Informations de copyright
Copyright © 2020. Published by Elsevier B.V.