Novel clearance of muscle proteins by muscle cells.
Clearance
Endocytosis
Muscle
Myoglobin
Troponin
Journal
European journal of cell biology
ISSN: 1618-1298
Titre abrégé: Eur J Cell Biol
Pays: Germany
ID NLM: 7906240
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
27
01
2020
revised:
11
10
2020
accepted:
29
10
2020
pubmed:
10
11
2020
medline:
4
8
2021
entrez:
9
11
2020
Statut:
ppublish
Résumé
Blood levels of cardiac troponins (cTn) and myoglobin are analysed when myocardial infarction (MI) is suspected. Here we describe a novel clearance mechanism for muscle proteins by muscle cells. The complete plasma clearance profile of cTn and myoglobin was followed in rats after intravenous or intermuscular injections and analysed by PET and fluorescence microscopy of muscle biopsies and muscle cells. Compared with intravenous injections, only 5 % of cTnT, 0.6 % of cTnI and 8 % of myoglobin were recovered in the circulation following intramuscular injection. In contrast, 47 % of the renal filtration marker FITC-sinistrin and 81 % of cTn fragments from MI-patients were recovered after intramuscular injection. In addition, PET and biopsy analysis revealed that cTn was taken up by the quadriceps muscle and both cTn and myoglobin were endocytosed by cultured muscle cells. This local clearance mechanism could possibly be the dominant clearance mechanism for cTn, myoglobin and other muscle damage biomarkers released by muscle cells.
Identifiants
pubmed: 33162173
pii: S0171-9335(20)30066-2
doi: 10.1016/j.ejcb.2020.151127
pii:
doi:
Substances chimiques
Muscle Proteins
0
Tnni3k protein, mouse
EC 2.7.-
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
151127Informations de copyright
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