Toward a comprehensive study for multielemental quantitative LA-ICP MS bioimaging in soft tissues.
Bioimaging
LA-ICP MS
Multielemental
Polymer films
Quantification
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
15 Jan 2021
15 Jan 2021
Historique:
received:
28
04
2020
revised:
07
08
2020
accepted:
10
08
2020
entrez:
10
11
2020
pubmed:
11
11
2020
medline:
15
5
2021
Statut:
ppublish
Résumé
Quantitative localization of metals in biological tissue sections is critical to obtain insight into metal toxicity mechanisms or their beneficial characteristics. This study presents the development of a quantitative LA-ICP MS bioimaging methodology based on the polymer film strategy and internal standardization. To maximize the number of elements mapped, an aqueous soluble polymer (dextran) was selected. Among the elements studied, the great majority (eight out eleven), i.e., Co, Ni, Cu, Zn, Se, Mo, Cd and Pt, exhibited linear regression after LA-ICP MS analysis of metal-spiked polymer standards. Methodology performances were carefully assessed as a function of the three internal standards (In, Rh and Ir) considered, the analytical operational conditions (ICP power, addition of O
Identifiants
pubmed: 33167245
pii: S0039-9140(20)30828-6
doi: 10.1016/j.talanta.2020.121537
pii:
doi:
Substances chimiques
Metals
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
121537Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.