Intermittent PTH Administration Increases Bone-Specific Blood Vessels and Surrounding Stromal Cells in Murine Long Bones.
Alkaline Phosphatase
/ metabolism
Animals
Blood Vessels
/ growth & development
Bone and Bones
/ blood supply
Male
Mice
Osteoblasts
Parathyroid Hormone
/ administration & dosage
Proto-Oncogene Proteins c-kit
/ metabolism
Receptor, Parathyroid Hormone, Type 1
/ metabolism
Stromal Cells
/ cytology
Vascular Endothelial Growth Factor A
/ metabolism
Vascular Endothelial Growth Factor Receptor-2
/ metabolism
Blood vessel
Bone
Endomucin
Parathyroid hormone (PTH)
Vascular smooth muscle cell
Journal
Calcified tissue international
ISSN: 1432-0827
Titre abrégé: Calcif Tissue Int
Pays: United States
ID NLM: 7905481
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
07
07
2020
accepted:
29
10
2020
pubmed:
11
11
2020
medline:
30
7
2021
entrez:
10
11
2020
Statut:
ppublish
Résumé
To verify whether PTH acts on bone-specific blood vessels and on cells surrounding these blood vessels, 6-week-old male mice were subjected to vehicle (control group) or hPTH [1-34] (20 µg/kg/day, PTH group) injections for 2 weeks. Femoral metaphyses were used for histochemical and immunohistochemical studies. In control metaphyses, endomucin-positive blood vessels were abundant, but αSMA-reactive blood vessels were scarce. In the PTH-administered mice, the lumen of endomucin-positive blood vessels was markedly enlarged. Moreover, many αSMA-positive cells were evident near the blood vessels, and seemed to derive from those vessels. These αSMA-positive cells neighboring the blood vessels showed features of mesenchymal stromal cells, such as immunopositivity for c-kit and tissue nonspecific alkaline phosphatase (TNALP). Thus, PTH administration increased the population of perivascular/stromal cells positive for αSMA and c-kit, which were likely committed to the osteoblastic lineage. To understand the cellular events that led to increased numbers and size of bone-specific blood vessels, we performed immunohistochemical studies for PTH/PTHrP receptor and VEGF. After PTH administration, PTH/PTHrP receptor, VEGF and its receptor flk-1 were consistently identified in both osteoblasts and blood vessels (endothelial cells and surrounding perivascular cells). Our findings suggest that exogenous PTH increases the number and size of bone-specific blood vessels while fostering perivascular/stromal cells positive for αSMA/TNALP/c-kit.
Identifiants
pubmed: 33170307
doi: 10.1007/s00223-020-00776-2
pii: 10.1007/s00223-020-00776-2
doi:
Substances chimiques
Parathyroid Hormone
0
Receptor, Parathyroid Hormone, Type 1
0
Vascular Endothelial Growth Factor A
0
vascular endothelial growth factor A, mouse
0
Proto-Oncogene Proteins c-kit
EC 2.7.10.1
Vascular Endothelial Growth Factor Receptor-2
EC 2.7.10.1
ALPL protein, mouse
EC 3.1.3.1
Alkaline Phosphatase
EC 3.1.3.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
391-406Références
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