Seroprevalence of SARS-CoV-2 antibodies in children: a prospective multicentre cohort study.


Journal

Archives of disease in childhood
ISSN: 1468-2044
Titre abrégé: Arch Dis Child
Pays: England
ID NLM: 0372434

Informations de publication

Date de publication:
07 2021
Historique:
received: 27 08 2020
accepted: 12 10 2020
pubmed: 12 11 2020
medline: 30 6 2021
entrez: 11 11 2020
Statut: ppublish

Résumé

Studies based on molecular testing of oral/nasal swabs underestimate SARS-CoV-2 infection due to issues with test sensitivity, test timing and selection bias. The objective of this study was to report the presence of SARS-CoV-2 antibodies, consistent with previous infection. This multicentre observational cohort study, conducted between 16 April to 3 July 2020 at 5 UK sites, recruited children of healthcare workers, aged 2-15 years. Participants provided blood samples for SARS-CoV-2 antibody testing and data were gathered regarding unwell contacts and symptoms. 1007 participants were enrolled, and 992 were included in the final analysis. The median age of participants was 10·1 years. There were 68 (6.9%) participants with positive SARS-CoV-2 antibody tests indicative of previous SARS-CoV-2 infection. Of these, 34/68 (50%) reported no symptoms prior to testing. The presence of antibodies and the mean antibody titre was not influenced by age. Following multivariable analysis four independent variables were identified as significantly associated with SARS-CoV-2 seropositivity: known infected household contact OR=10.9 (95% CI 6.1 to 19.6); fatigue OR=16.8 (95% CI 5.5 to 51.9); gastrointestinal symptoms OR=6.6 (95% CI 3.0 to 13.8); and changes in sense of smell or taste OR=10.0 (95% CI 2.4 to 11.4). Children demonstrated similar antibody titres in response to SARS-CoV-2 irrespective of age. Fatigue, gastrointestinal symptoms and changes in sense of smell or taste were the symptoms most strongly associated with SARS-CoV-2 antibody positivity. NCT0434740.

Sections du résumé

BACKGROUND
Studies based on molecular testing of oral/nasal swabs underestimate SARS-CoV-2 infection due to issues with test sensitivity, test timing and selection bias. The objective of this study was to report the presence of SARS-CoV-2 antibodies, consistent with previous infection.
DESIGN
This multicentre observational cohort study, conducted between 16 April to 3 July 2020 at 5 UK sites, recruited children of healthcare workers, aged 2-15 years. Participants provided blood samples for SARS-CoV-2 antibody testing and data were gathered regarding unwell contacts and symptoms.
RESULTS
1007 participants were enrolled, and 992 were included in the final analysis. The median age of participants was 10·1 years. There were 68 (6.9%) participants with positive SARS-CoV-2 antibody tests indicative of previous SARS-CoV-2 infection. Of these, 34/68 (50%) reported no symptoms prior to testing. The presence of antibodies and the mean antibody titre was not influenced by age. Following multivariable analysis four independent variables were identified as significantly associated with SARS-CoV-2 seropositivity: known infected household contact OR=10.9 (95% CI 6.1 to 19.6); fatigue OR=16.8 (95% CI 5.5 to 51.9); gastrointestinal symptoms OR=6.6 (95% CI 3.0 to 13.8); and changes in sense of smell or taste OR=10.0 (95% CI 2.4 to 11.4).
DISCUSSION
Children demonstrated similar antibody titres in response to SARS-CoV-2 irrespective of age. Fatigue, gastrointestinal symptoms and changes in sense of smell or taste were the symptoms most strongly associated with SARS-CoV-2 antibody positivity.
TRIAL REGISTRATION NUMBER
NCT0434740.

Identifiants

pubmed: 33172887
pii: archdischild-2020-320558
doi: 10.1136/archdischild-2020-320558
doi:

Substances chimiques

Antibodies, Viral 0

Banques de données

ClinicalTrials.gov
['NCT04347408']

Types de publication

Journal Article Multicenter Study Observational Study Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

680-686

Informations de copyright

© Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.

Déclaration de conflit d'intérêts

Competing interests: None declared.

Auteurs

Thomas Waterfield (T)

Queen's University Belfast Wellcome-Wolfson Institute for Experimental Medicine, Belfast, UK thomas.waterfield@googlemail.com.
Emergency Department, Children's Health Ireland at Temple Street, Dublin D01 YC67, Ireland.

Chris Watson (C)

Queen's University Belfast Wellcome-Wolfson Institute for Experimental Medicine, Belfast, UK.

Rebecca Moore (R)

General Paediatrics, Royal Belfast Hospital for Sick Children, Belfast, UK.

Kathryn Ferris (K)

Belfast Health and Social Care Trust, Belfast, UK.

Claire Tonry (C)

Queen's University Belfast Wellcome-Wolfson Institute for Experimental Medicine, Belfast, UK.

Alison Watt (A)

Regional Virus Laboratory, Belfast Health and Social Care Trust, Belfast, UK.

Claire McGinn (C)

General Paediatrics, Royal Belfast Hospital for Sick Children, Belfast, UK.

Steven Foster (S)

Emergency Department, Royal Hospital for Children, Glasgow, UK.

Jennifer Evans (J)

Cardiff and Vale University Health Board, Cardiff, UK.

Mark David Lyttle (MD)

Emergency Department, University Hospitals Bristol NHS Foundation Trust, Bristol, UK.
Health and Applied Sciences, University of the West of England, Bristol, UK.

Shazaad Ahmad (S)

Department of Virology, Manchester Medical Microbiology Partnership, Manchester University NHS Foundation Trust, Manchester, Greater Manchester, UK.
Manchester Academic Health Science Centre, Manchester, UK.

Shamez Ladhani (S)

Immunisation and Countermeasures Division, Public Health England, London, UK.

Michael Corr (M)

Belfast Health and Social Care Trust, Belfast, UK.

Lisa McFetridge (L)

Mathematical Sciences Research Centre, Queen's University Belfast, Belfast, UK.

Hannah Mitchell (H)

Mathematical Sciences Research Centre, Queen's University Belfast, Belfast, UK.

Kevin Brown (K)

Virus Reference Department, Public Health England, Colindale, UK.

Gayatri Amirthalingam (G)

Immunisation,Hepatitis & Blood Safety Department, Public Health England Immunisation and Countermeasures Division, London, UK.

Julie-Ann Maney (JA)

Emergency Department, Royal Belfast Hospital for Sick Children, Belfast, UK.

Sharon Christie (S)

Paediatric Infectious Diseases, Royal Belfast Hospital for Sick Children, Belfast, UK.

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Classifications MeSH