Multicenter Study on Differential Human Neutrophil Antigen 2 Expression and Underlying Molecular Mechanisms.

Human neutrophil antigens Molecular analysis Monoallelic expression Neutropenia

Journal

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie
ISSN: 1660-3796
Titre abrégé: Transfus Med Hemother
Pays: Switzerland
ID NLM: 101176417

Informations de publication

Date de publication:
Oct 2020
Historique:
received: 28 08 2019
accepted: 18 12 2019
entrez: 11 11 2020
pubmed: 12 11 2020
medline: 12 11 2020
Statut: ppublish

Résumé

The human neutrophil antigen 2 (HNA-2), which is expressed on CD177, is undetectable in 3-5% of the normal population. Exposure of these HNA-2 An international collaboration was conducted on the genetic analysis of HNA-2-phenotyped blood samples, including HNA-2-deficient individuals, mothers, and the respective children with neonatal immune neutropenia and regular blood donors. From a total of 54 HNA-2 This comprehensive study clearly demonstrates the impact of single nucleotide polymorphisms on the expression of HNA-2 on the neutrophil surface but challenges the hypothesis of regulatory epigenetic effects being implicated in the bimodal CD177 expression pattern.

Sections du résumé

BACKGROUND BACKGROUND
The human neutrophil antigen 2 (HNA-2), which is expressed on CD177, is undetectable in 3-5% of the normal population. Exposure of these HNA-2
STUDY DESIGN METHODS
An international collaboration was conducted on the genetic analysis of HNA-2-phenotyped blood samples, including HNA-2-deficient individuals, mothers, and the respective children with neonatal immune neutropenia and regular blood donors.
RESULTS RESULTS
From a total of 54 HNA-2
CONCLUSION CONCLUSIONS
This comprehensive study clearly demonstrates the impact of single nucleotide polymorphisms on the expression of HNA-2 on the neutrophil surface but challenges the hypothesis of regulatory epigenetic effects being implicated in the bimodal CD177 expression pattern.

Identifiants

pubmed: 33173457
doi: 10.1159/000505523
pii: tmh-0047-0385
pmc: PMC7590758
doi:

Types de publication

Journal Article

Langues

eng

Pagination

385-395

Informations de copyright

Copyright © 2020 by S. Karger AG, Basel.

Déclaration de conflit d'intérêts

The authors disclose no conflicts of interest.

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Auteurs

Brigitte K Flesch (BK)

German Red Cross Blood Service Rhineland-Palatinate and Saarland, Bad Kreuznach, Germany.
German Red Cross Blood Service West, Hagen, Germany.

Angelika Reil (A)

German Red Cross Blood Service West, Hagen, Germany.

Núria Nogués (N)

Immunohematology Laboratory, Blood and Tissue Bank, Barcelona, Spain.

Carme Canals (C)

Immunohematology Laboratory, Blood and Tissue Bank, Barcelona, Spain.

Peter Bugert (P)

Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Service of Baden-Württemberg, Hessen gGmbH, Mannheim, Germany.

Torsten J Schulze (TJ)

Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Service of Baden-Württemberg, Hessen gGmbH, Mannheim, Germany.
Institute Springe, German Red Cross Blood Service NSTOB, Springe, Germany.

Elly Huiskes (E)

Department of Immunohematology Diagnostics, Sanquin, Amsterdam, The Netherlands.

Leendert Porcelijn (L)

Department of Immunohematology Diagnostics, Sanquin, Amsterdam, The Netherlands.

Petter Höglund (P)

Center for Hematology and Regenerative Medicine, Karolinska University Hospital, Stockholm, Sweden.

Paul Ratcliffe (P)

Center for Hematology and Regenerative Medicine, Karolinska University Hospital, Stockholm, Sweden.

Marlies Schönbacher (M)

Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Vienna, Austria.

Hans Kerchrom (H)

Centre Hospitalier Universitaire de Nantes, Nantes, France.

Josina Kellershohn (J)

Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany.

Behnaz Bayat (B)

Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany.

Classifications MeSH