Multiscale Characterization of Embryonic Long Bone Mineralization in Mice.
Fourier transform infra‐red microspectroscopy
X‐ray fluorescence spectroscopy
X‐ray tomography
bone development
small‐ and wide‐angle X‐ray scattering
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
03
07
2020
entrez:
11
11
2020
pubmed:
12
11
2020
medline:
12
11
2020
Statut:
epublish
Résumé
Long bone mineralization occurs through endochondral ossification, where a cartilage template mineralizes into bone-like tissue with a hierarchical organization from the whole bone-scale down to sub-nano scale. Whereas this process has been extensively studied at the larger length scales, it remains unexplored at some of the smaller length scales. In this study, the changes in morphology, composition, and structure during embryonic mineralization of murine humeri are investigated using a range of high-resolution synchrotron-based imaging techniques at several length scales. With micro- and nanometer spatial resolution, the deposition of elements and the shaping of mineral platelets are followed. Rapid mineralization of the humeri occurs over approximately four days, where mineral to matrix ratio and calcium content in the most mineralized zone reach adult values shortly before birth. Interestingly, zinc is consistently found to be localized at the sites of ongoing new mineralization. The mineral platelets in the most recently mineralized regions are thicker, longer, narrower, and less aligned compared to those further into the mineralized region. In summary, this study demonstrates a specific spatial distribution of zinc, with highest concentration where new mineral is being deposited and that the newly formed mineral platelets undergo slight reshaping and reorganization during embryonic development.
Identifiants
pubmed: 33173750
doi: 10.1002/advs.202002524
pii: ADVS2027
pmc: PMC7610310
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2002524Informations de copyright
© 2020 The Authors. Published by Wiley‐VCH GmbH.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Bone. 2013 Nov;57(1):184-93
pubmed: 23932972
Biomaterials. 2007 May;28(15):2465-78
pubmed: 17175021
PLoS One. 2012;7(1):e29258
pubmed: 22272230
J Struct Biol. 2011 Jun;174(3):527-35
pubmed: 21440636
J Bone Miner Metab. 2020 May;38(3):289-298
pubmed: 31807903
Analyst. 2010 Dec;135(12):3147-55
pubmed: 21038039
J Bone Miner Res. 2002 Jun;17(6):1118-26
pubmed: 12054168
Appl Spectrosc. 2011 Jun;65(6):595-603
pubmed: 21639980
J Biol Chem. 1988 Dec 5;263(34):18513-9
pubmed: 3192545
J Orthop Res. 2010 Dec;28(12):1626-33
pubmed: 20540098
J Struct Biol. 2011 Nov;176(2):159-67
pubmed: 21855638
Acta Biomater. 2018 Mar 15;69:323-331
pubmed: 29410089
Calcif Tissue Int. 2010 Apr;86(4):294-306
pubmed: 20221590
Eur Cell Mater. 2008 Apr 01;15:53-76
pubmed: 18382990
J Med Food. 2009 Feb;12(1):118-23
pubmed: 19298204
Annu Rev Cell Dev Biol. 2000;16:191-220
pubmed: 11031235
Calcif Tissue Int. 2016 Jul;99(1):76-87
pubmed: 26914607
J Struct Biol. 2016 Sep;195(3):337-344
pubmed: 27417019
Trends Cell Biol. 2004 Feb;14(2):86-93
pubmed: 15102440
J Bone Miner Metab. 2016 Jan;34(1):41-50
pubmed: 25773047
Bone. 2020 Jan;130:115086
pubmed: 31669250
J Biomed Opt. 2014 Feb;19(2):025003
pubmed: 24522802
Nature. 2003 May 15;423(6937):332-6
pubmed: 12748651
Matrix Biol. 2016 Mar;50:1-15
pubmed: 26454027
J Bone Miner Res. 2010 Jun;25(6):1360-6
pubmed: 20200925
Science. 2018 May 4;360(6388):
pubmed: 29724924
Calcif Tissue Int. 2013 May;92(5):418-28
pubmed: 23380987
Adv Sci (Weinh). 2020 Sep 24;7(21):2002524
pubmed: 33173750
J Bone Miner Res. 2001 Oct;16(10):1821-8
pubmed: 11585346
ACS Cent Sci. 2019 Aug 28;5(8):1449-1460
pubmed: 31482128
Appl Radiat Isot. 2010 Apr-May;68(4-5):730-4
pubmed: 19836249
Hear Res. 2020 Jun;391:107948
pubmed: 32283439
Proc Natl Acad Sci U S A. 2017 Oct 3;114(40):10542-10547
pubmed: 28923958
ACS Nano. 2019 Nov 26;13(11):12949-12956
pubmed: 31613594
Osteoporos Int. 2009 Jun;20(6):1057-63
pubmed: 19340510
Calcif Tissue Int. 2018 Dec;103(6):606-616
pubmed: 30008091
Calcif Tissue Int. 1998 Apr;62(4):341-9
pubmed: 9504960
Calcif Tissue Int. 1991 Oct;49(4):251-8
pubmed: 1760769
Med Eng Phys. 1998 Mar;20(2):92-102
pubmed: 9679227
J Bone Miner Res. 2007 Jul;22(7):1037-45
pubmed: 17402847
Development. 2016 Nov 1;143(21):3933-3943
pubmed: 27621060
Biochim Biophys Acta. 2001 Jul 2;1527(1-2):11-9
pubmed: 11420138
J Struct Biol. 2016 Jul;195(1):82-92
pubmed: 27108185
Bone. 2016 Jun;87:147-58
pubmed: 27072517
Biofactors. 2014 Jul-Aug;40(4):425-35
pubmed: 24615876
Eur Cell Mater. 2020 Feb 27;39:136-155
pubmed: 32103474
Bone. 2006 Sep;39(3):530-41
pubmed: 16769265
Acta Biomater. 2015 Aug;22:92-102
pubmed: 25829108
Bone. 2010 May;46(5):1275-85
pubmed: 19948261
Bone. 2001 Dec;29(6):565-70
pubmed: 11728928
Stem Cell Res Ther. 2014 Dec 29;5(6):144
pubmed: 25689288