Optimal sampling strategies for darunavir and external validation of the underlying population pharmacokinetic model.


Journal

European journal of clinical pharmacology
ISSN: 1432-1041
Titre abrégé: Eur J Clin Pharmacol
Pays: Germany
ID NLM: 1256165

Informations de publication

Date de publication:
Apr 2021
Historique:
received: 12 08 2020
accepted: 31 10 2020
pubmed: 12 11 2020
medline: 25 2 2023
entrez: 11 11 2020
Statut: ppublish

Résumé

A variety of diagnostic methods are available to validate the performance of population pharmacokinetic models. Internal validation, which applies these methods to the model building dataset and to additional data generated through Monte Carlo simulations, is often sufficient, but external validation, which requires a new dataset, is considered a more rigorous approach, especially if the model is to be used for predictive purposes. Our first objective was to validate a previously published population pharmacokinetic model of darunavir, an HIV protease inhibitor boosted with ritonavir or cobicistat. Our second objective was to use this model to derive optimal sampling strategies that maximize the amount of information collected with as few pharmacokinetic samples as possible. A validation dataset comprising 164 sparsely sampled individuals using ritonavir-boosted darunavir was used for validation. Standard plots of predictions and residuals, NPDE, visual predictive check, and bootstrapping were applied to both the validation set and the combined learning/validation set in NONMEM to assess model performance. D-optimal designs for darunavir were then calculated in PopED and further evaluated in NONMEM through simulations. External validation confirmed model robustness and accuracy in most scenarios but also highlighted several limitations. The best one-, two-, and three-point sampling strategies were determined to be pre-dose (0 h); 0 and 4 h; and 1, 4, and 19 h, respectively. A combination of samples at 0, 1, and 4 h was comparable to the optimal three-point strategy. These could be used to reliably estimate individual pharmacokinetic parameters, although with fewer samples, precision decreased and the number of outliers increased significantly. Optimal sampling strategies derived from this model could be used in clinical practice to enhance therapeutic drug monitoring or to conduct additional pharmacokinetic studies.

Identifiants

pubmed: 33175180
doi: 10.1007/s00228-020-03036-2
pii: 10.1007/s00228-020-03036-2
pmc: PMC7935830
doi:

Substances chimiques

HIV Protease Inhibitors 0
Darunavir YO603Y8113

Types de publication

Journal Article Validation Study

Langues

eng

Sous-ensembles de citation

IM

Pagination

607-616

Subventions

Organisme : Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture (FRIA)
ID : FC16749

Références

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Auteurs

Gabriel Stillemans (G)

Integrated PharmacoMetrics, PharmacoGenomics and PharmacoKinetics, Louvain Drug Research Institute, Université catholique de Louvain, Avenue E. Mounier 72, B01.72.0, Brussels, Belgium. gabriel.stillemans@uclouvain.be.
Louvain Centre for Toxicology and Applied Pharmacology, Institut de recherche expérimentale et clinique, Université catholique de Louvain, Brussels, Belgium. gabriel.stillemans@uclouvain.be.

Leila Belkhir (L)

Louvain Centre for Toxicology and Applied Pharmacology, Institut de recherche expérimentale et clinique, Université catholique de Louvain, Brussels, Belgium.
AIDS Reference Center, Department of Internal Medicine, Cliniques universitaires Saint-Luc, Université catholique de Louvain, Brussels, Belgium.

Bernard Vandercam (B)

AIDS Reference Center, Department of Internal Medicine, Cliniques universitaires Saint-Luc, Université catholique de Louvain, Brussels, Belgium.

Anne Vincent (A)

AIDS Reference Center, Department of Internal Medicine, Cliniques universitaires Saint-Luc, Université catholique de Louvain, Brussels, Belgium.

Vincent Haufroid (V)

Louvain Centre for Toxicology and Applied Pharmacology, Institut de recherche expérimentale et clinique, Université catholique de Louvain, Brussels, Belgium.
Department of Clinical Chemistry, Cliniques universitaires Saint-Luc, Brussels, Belgium.

Laure Elens (L)

Integrated PharmacoMetrics, PharmacoGenomics and PharmacoKinetics, Louvain Drug Research Institute, Université catholique de Louvain, Avenue E. Mounier 72, B01.72.0, Brussels, Belgium.
Louvain Centre for Toxicology and Applied Pharmacology, Institut de recherche expérimentale et clinique, Université catholique de Louvain, Brussels, Belgium.

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