Evaluating the effects of vacuum on the microstructure and biocompatibility of bovine decellularized pericardium.
acellular
bovine
extracellular matrix
pericardium
porosity
vacuum
Journal
Journal of tissue engineering and regenerative medicine
ISSN: 1932-7005
Titre abrégé: J Tissue Eng Regen Med
Pays: England
ID NLM: 101308490
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
21
02
2020
revised:
27
08
2020
accepted:
04
11
2020
pubmed:
12
11
2020
medline:
15
12
2021
entrez:
11
11
2020
Statut:
ppublish
Résumé
The aim of this study was evaluating the effects of vacuum on microstructure and biocompatibility of bovine decellularized pericardium. So the bovine pericardia were decellularized and then the vacuum was applied for two periods of time; 90 and 180 min. DNA, glucose amino glycan, collagen and elastin content assay, scanning electron microscopy (SEM) examination, hematoxylin and eosin (H&E) and Masson's trichrome stainings performed to evaluate microstructure of tissues. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, subcutaneous implantation, and tensile test were used to assay biocompatibility and mechanical properties of decellularized tissues. The results showed that applying vacuum reduced residual DNA significantly. Vacuum after 180 min reduced more residual DNA. There were no significant differences in the content of glucose amino glycan (GAG), collagen, and elastin between the vacuumed and control groups. SEM examination was revealed that vacuum for 180 min increased pore size and porosity more than 90 min and control groups. H&E and Masson's trichrome stainings revealed extracellular matrix preservation after decellularization in all groups. Cell viability was increased in vacuumed samples significantly after 72 h in vaccumed samples. H&E staining and tensile test after implantation of tissues were showed less inflammation in the vacuum applied tissues and increased durability. The vacuum increased DNA removal, pore size, porosity, and biocompatibility in vitro and in vivo and durability of bovine decellularized pericardium in vivo. Considering the important role of time, more studies should be performed to optimize time, intensity, and method of application of vacuum in decellularization of different tissues as well as bovine pericardium.
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
116-128Informations de copyright
© 2020 John Wiley & Sons Ltd.
Références
Alizadeh, M., Rezakhani, L., Soleimannejad, M., Sharifi, E., Anjomshoa, M., Alizadeh, A. (2019). Evaluation of vacuum washing in the removal of SDS from decellularized bovine pericardium: Method and device description. Heliyon, 5, e02253.
Almine, J. F., Wise, S. G., Weiss, A. S. (2012). Elastin signaling in wound repair. Birth Defects Research Part C: Embryo Today: Reviews, 96, 248-257.
Annabi, N., Nichol, J. W., Zhong, X., Ji, C., Koshy, S., Khademhosseini, A., Dehghani, F. (2010). Controlling the porosity and microarchitecture of hydrogels for tissue engineering. Tissue Engineering Part B: Reviews, 16, 371-383.
Ayala, P. Dai, E. Hawes, M. Liu, L. Chaudhuri, O. Haller, C. A., … Chaikof, E. L. (2018). Evaluation of a bioengineered construct for tissue engineering applications. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 106, 2345-2354.
Badylak, S. F., Taylor, D., Uygun, K. (2011). Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annual Review of Biomedical Engineering, 13, 27-53.
Butler, C. R. Hynds, R. E., Crowley, C., Gowers, K. H., Partington, L., Hamilton, N. J., … Urbani, L. (2017). Vacuum-assisted decellularization: An accelerated protocol to generate tissue-engineered human tracheal scaffolds. Biomaterials, 124, 95-105.
Cen, L., Liu, W., Cui, L., Zhang, W., Cao, Y. (2008). Collagen tissue engineering: development of novel biomaterials and applications. Pediatric Research, 63, 492.
Chang, Y., Tsai, C.-C., Liang, H.-C., Sung, H.-W. (2002). In vivo evaluation of cellular and acellular bovine pericardia fixed with a naturally occurring crosslinking agent (genipin). Biomaterials 23, 2447-2457.
Cunniffe, G. M., Vinardell, T., Murphy, J. M., Thompson, E. M., Matsiko, A., O'Brien, F. J., Kelly, D. J. (2015). Porous decellularized tissue engineered hypertrophic cartilage as a scaffold for large bone defect healing. Acta Biomaterialia, 23, 82-90.
Daamen, W. F., Veerkamp, J., Van Hest, J., Van Kuppevelt, T. (2007). Elastin as a biomaterial for tissue engineering. Biomaterials, 28, 4378-4398.
den Bakker, M. (2017). Is histopathology still the gold standard? Nederlands Tijdschrift Voor Geneeskunde, 160, D981.
Dong, J., Li, Y., Mo, X. (2013). The study of a new detergent (octyl-glucopyranoside) for decellularizing porcine pericardium as tissue engineering scaffold. Journal of Surgical Research, 183, 56-67.
Dong, X, Wei, X., Yi, W., Gu, C., Kang, X., Liu, Y., … Yi, D. (2009). RGD-modified acellular bovine pericardium as a bioprosthetic scaffold for tissue engineering. Journal of Materials Science: Materials in Medicine, 20(11), 2327.
Fazio, M. J, Mattei, M. G., Passage, E., Chu, M. L., Black, D., Solomon, E., … Uitto, J. (1991). Human elastin gene: New evidence for localization to the long arm of chromosome 7. American Journal of Human Genetics, 48(4), 696.
Feng, B, Jinkang, Z., Zhen, W., Jianxi, L., Jiang, C., Jian, L., … Xin, D. (2011). The effect of pore size on tissue ingrowth and neovascularization in porous bioceramics of controlled architecture in vivo. Biomedical Materials, 6(1), 015007.
Fu, R. H., Wang, Y. C., Liu, S. P., Shih, T. R., Lin, H. L., Chen, Y. M., … Huang, S. J. (2014). Decellularization and recellularization technologies in tissue engineering. Cell Transplantation, 23(4-5), 621-630.
Gilbert, T. W. (2012). Strategies for tissue and organ decellularization. Journal of Cellular Biochemistry, 113, 2217-2222.
Gilbert, T. W., Freund, J. M., & Badylak, S. F. (2009). Quantification of DNA in biologic scaffold materials. Journal of Surgical Research, 152, 135-139.
Gillies, A. R., Smith, L. R., Lieber, R. L., & Varghese, S. (2010). Method for decellularizing skeletal muscle without detergents or proteolytic enzymes. Tissue Engineering Part C: Methods, 17, 383-389.
Heuschkel, M. A. Leitolis, A., Roderjan, J. G., Suss, P. H., Luzia, C. A. O., da Costa, F. D. A., … Stimamiglio, M. A. (2019). In vitro evaluation of bovine pericardium after a soft decellularization approach for use in tissue engineering. Xenotransplantation, 26(2), e12464.
Hodde, J. (2002). Naturally occurring scaffolds for soft tissue repair and regeneration. Tissue Engineering, 8, 295-308.
Hong, P. (2016). Development and characterization of decellularized rabbit tracheal cartilage matrix for use in tissue engineering. Nova Scotia, Canada: Dalhousie University Halifax.
Karimpour Malekshah, A. Talebpour Amiri, F., Ghaffari, E., Alizadeh, A., Jamalpoor, Z., Mirhosseini, M., … Barzegarnejad, A. (2016). Growth and chondrogenic differentiation of mesenchymal stem cells derived from human adipose tissue on chitosan scaffolds. Journal of Babol University of Medical Sciences, 18(9), 32-38.
Keane, T. J., Swinehart, I. T., & Badylak, S. F. (2015). Methods of tissue decellularization used for preparation of biologic scaffolds and in vivo relevance. Methods, 84, 25-34.
Khademhosseini, A., & Langer, R. (2007). Microengineered hydrogels for tissue engineering. Biomaterials, 28, 5087-5092.
Khan, M. A. A., Chipp, E., Hardwicke, J., Srinivasan, K., Shaw, S., & Rayatt, S. (2010). The use of Dermal Regeneration Template (Integra®) for reconstruction of a large full-thickness scalp and calvarial defect with exposed dura. Journal of Plastic, Reconstructive & Aesthetic Surgery, 63, 2168-2171.
Klenke, F. M., Liu, Y., Yuan, H., Hunziker, E. B., Siebenrock, K. A., & Hofstetter, W. (2008). Impact of pore size on the vascularization and osseointegration of ceramic bone substitutes in vivo. Journal of Biomedical Materials Research Part A, 85, 777-786.
Lange, P. Greco, K., Partington, L., Carvalho, C., Oliani, S., Birchall, M. A., … Ansari, T. (2017). Pilot study of a novel vacuum-assisted method for decellularization of tracheae for clinical tissue engineering applications. Journal of Tissue Engineering and Regenerative Medicine, 11(3), 800-811.
Li, N., Li, Y., Gong, D., Xia, C., Liu, X., & Xu, Z. (2018). Efficient decellularization for bovine pericardium with extracellular matrix preservation and good biocompatibility. Interactive Cardiovascular and Thoracic Surgery, 26, 768-776.
Lowry, O. H., Gilligan, D. R., & Katersky, E. M. (1941). The determination of collagen and elastin in tissues, with results obtained in various normal tissues from different species. Journal of Biological Chemistry, 139, 795-804.
Lu, Q., Ganesan, K., Simionescu, D. T., & Vyavahare, N. R. (2004). Novel porous aortic elastin and collagen scaffolds for tissue engineering. Biomaterials, 25, 5227-5237.
Mathapati, S., Bishi, D. K., Guhathakurta, S., Cherian, K. M., Venugopal, J. R., Ramakrishna, S., & Verma, R. S. (2013). Biomimetic acellular detoxified glutaraldehyde cross-linked bovine pericardium for tissue engineering. Materials Science and Engineering: C, 33, 1561-1572.
Mendoza-Novelo, B., Avila, E. E., Cauich-Rodríguez, J. V., Jorge-Herrero, E., Rojo, F. J., Guinea, G. V., & Mata-Mata, J. L. (2011). Decellularization of pericardial tissue and its impact on tensile viscoelasticity and glycosaminoglycan content. Acta Biomaterialia, 7, 1241-1248.
Mirsadraee, S., Wilcox, H. E., Korossis, S. A., Kearney, J. N., Watterson, K. G., Fisher, J., & Ingham, E. (2006). Development and characterization of an acellular human pericardial matrix for tissue engineering. Tissue Engineering, 12, 763-773.
Park, S.-N., Lee, H. J., Lee, K. H., & Suh, H. (2003). Biological characterization of EDC-crosslinked collagen-hyaluronic acid matrix in dermal tissue restoration. Biomaterials, 24, 1631-1641.
Raman, R., Sasisekharan, V., & Sasisekharan, R. (2005). Structural insights into biological roles of protein-glycosaminoglycan interactions. Chemistry & Biology, 12, 267-277.
Rashtbar, M. Hadjati, J., Ai, J., Jahanzad, I., Azami, M., Shirian, S., … Sadroddiny, E. (2018). Characterization of decellularized ovine small intestine submucosal layer as extracellular matrix-based scaffold for tissue engineering. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 106(3), 933-944.
Rezakhani, L., Khazaei, M. R., Ghanbari, A., & Khazaei, M. (2017). Crab shell extract induces prostate cancer cell line (LNcap) apoptosis and decreases nitric oxide secretion. Cell Journal (Yakhteh), 19, 231.
Rijal, G. (2017). The decellularized extracellular matrix in regenerative medicine. Future Medicine, 12, 475-477.
Smart, N. J., Marshall, M., & Daniels, I. R. (2012). Biological meshes: A review of their use in abdominal wall hernia repairs. The Surgeon, 10, 159-171.
Song, M., Liu, Y., & Hui, L. (2018). Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments. Molecular Medicine Reports, 17, 138-146.
Swetha, M., Sahithi, K., Moorthi, A., Srinivasan, N., Ramasamy, K., & Selvamurugan, N. (2010). Biocomposites containing natural polymers and hydroxyapatite for bone tissue engineering. International Journal of Biological Macromolecules, 47, 1-4.
Villalona, G. A. Udelsman, B., Duncan, D. R., McGillicuddy, E., Sawh-Martinez, R. F., Hibino, N., … Breuer, C. K. (2010). Cell-seeding techniques in vascular tissue engineering. Tissue Engineering Part B: Reviews, 16(3), 341-350.
Wenger, S. (2012). Anesthesia and analgesia in rabbits and rodents. Journal of Exotic Pet Medicine, 21, 7-16.
Wiegmann, B., Zardo, P., Dickgreber, N., Länger, F., Fegbeutel, C., Haverich, A., & Fischer, S. (2010). Biological materials in chest wall reconstruction: Initial experience with the peri-guard repair Patch®. European Journal of Cardio-Thoracic Surgery, 37, 602-605.
Yang, S., Leong, K.-F., Du, Z., & Chua, C.-K. (2001). The design of scaffolds for use in tissue engineering. Part I. Traditional factors. Tissue Engineering, 7, 679-689.
Ye, Q., Zünd, G., Jockenhoevel, S., Hoerstrup, S. P., Schoeberlein, A., Grunenfelder, J., & Turina, M. (2000). Tissue engineering in cardiovascular surgery: New approach to develop completely human autologous tissue. European Journal of Cardio-Thoracic Surgery, 17, 449-454.
Yu, C., Bianco, J., Brown, C., Fuetterer, L., Watkins, J. F., Samani, A., & Flynn, L. E. (2013). Porous decellularized adipose tissue foams for soft tissue regeneration. Biomaterials, 34, 3290-3302.
Zeltinger, J., Sherwood, J. K., Graham, D. A., Müeller, R., & Griffith, L. G. (2001). Effect of pore size and void fraction on cellular adhesion. Proliferation, and Matrix Deposition Tissue Engineering, 7, 557-572.