Universal Single-Residue Terminal Labels for Fluorescent Live Cell Imaging of Microproteins.

Animals Fluorescent Dyes / chemistry HEK293 Cells Humans Microscopy, Fluorescence Mitochondrial Proteins / chemistry Neoplasm Proteins / chemistry Proteins / chemistry

Journal

Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056

Informations de publication

Date de publication:
25 11 2020
Historique:
pubmed: 12 11 2020
medline: 17 4 2021
entrez: 11 11 2020
Statut: ppublish

Résumé

Genetically encoded fluorescent tags for visualization of proteins in living cells add six to several hundred amino acids to the protein of interest. While suitable for most proteins, common tags easily match and exceed the size of microproteins of 60 amino acids or less. The added molecular weight and structure of such fluorescent tag may thus significantly affect

Identifiants

DOI: 10.1021/jacs.0c09574 PMID: 33175524
pubmed: 33175524
doi: 10.1021/jacs.0c09574
doi:

Substances chimiques

Fluorescent Dyes 0
Mitochondrial Proteins 0
Neoplasm Proteins 0
Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

20080-20087

Auteurs

Lorenzo Lafranchi (L)

Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, Karolinska Institutet, Stockholm, 17165, Sweden.
Ming Wai Lau Centre for Reparative Medicine, Stockholm node, Karolinska Institutet, Stockholm, 17165, Sweden.

Dörte Schlesinger (D)

Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, Karolinska Institutet, Stockholm, 17165, Sweden.
Ming Wai Lau Centre for Reparative Medicine, Stockholm node, Karolinska Institutet, Stockholm, 17165, Sweden.

Kyle J Kimler (KJ)

Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, Karolinska Institutet, Stockholm, 17165, Sweden.
Ming Wai Lau Centre for Reparative Medicine, Stockholm node, Karolinska Institutet, Stockholm, 17165, Sweden.

Simon J Elsässer (SJ)

Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, Karolinska Institutet, Stockholm, 17165, Sweden.
Ming Wai Lau Centre for Reparative Medicine, Stockholm node, Karolinska Institutet, Stockholm, 17165, Sweden.
ORCID: 0000-0001-8724-4849

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Universal Single-Residue Terminal Labels for...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Universal Single-Residue Terminal Labels for...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Universal Single-Residue Terminal Labels for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Universal Single-Residue Terminal Labels for...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › Universal Single-Residue Terminal Labels for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines mitochondriales › Universal Single-Residue Terminal Labels for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines tumorales › Universal Single-Residue Terminal Labels for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Universal Single-Residue Terminal Labels for...