In Vitro Meiosis of Male Germline Stem Cells.
Sertoli cell lines
in vitro spermatogenesis
meiosis
meiotic checkpoints
spermatogonial stem cells
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
10 11 2020
10 11 2020
Historique:
received:
26
05
2020
revised:
13
10
2020
accepted:
14
10
2020
entrez:
11
11
2020
pubmed:
12
11
2020
medline:
8
9
2021
Statut:
ppublish
Résumé
In vitro spermatogenesis has been achieved by culturing mouse embryonic stem cells (ESCs) together with a cell suspension of male juvenile gonad. However, for human fertility treatment or preservation, patient-specific ESCs or juvenile gonad is not available. We therefore aim to achieve in vitro spermatogenesis using male germline stem cells (GSCs) without the use of juvenile gonad. GSCs, when cultured on immortalized Sertoli cells, were able to enter meiosis, reach the meiotic metaphase stages, and sporadically form spermatid-like cells. However, the in vitro-formed pachytene-like spermatocytes did not display full chromosome synapsis and did not form meiotic crossovers. Despite this, the meiotic checkpoints that usually eliminate such cells to prevent genomic instabilities from being transmitted to the offspring were not activated, allowing the cells to proceed to the meiotic metaphase stages. In vitro-generated spermatid-like cells should thus be thoroughly investigated before being considered for clinical use.
Identifiants
pubmed: 33176123
pii: S2213-6711(20)30416-1
doi: 10.1016/j.stemcr.2020.10.006
pmc: PMC7664054
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1140-1153Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
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