First identification of Anaplasma phagocytophilum in both a biting tick Ixodes nipponensis and a patient in Korea: a case report.
Aged, 80 and over
Anaplasma phagocytophilum
/ genetics
Anaplasmosis
/ diagnosis
Animals
Anti-Bacterial Agents
/ administration & dosage
Doxycycline
/ administration & dosage
Female
Fever
HL-60 Cells
Humans
Ixodes
/ microbiology
Polymerase Chain Reaction
RNA, Ribosomal, 16S
/ genetics
Republic of Korea
Tick Bites
/ microbiology
Tick-Borne Diseases
/ diagnosis
Treatment Outcome
Anaplasma phagocytophilum
Human granulocytic anaplasmosis
Ixodes nipponensis
Tick bites
Journal
BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551
Informations de publication
Date de publication:
11 Nov 2020
11 Nov 2020
Historique:
received:
06
05
2020
accepted:
16
10
2020
entrez:
12
11
2020
pubmed:
13
11
2020
medline:
20
11
2020
Statut:
epublish
Résumé
Human granulocytic anaplasmosis (HGA) is a tick-borne infectious disease caused by Anaplasma phagocytophilum. To date, there have been no reported cases of A. phagocytophilum infection found in both the biting tick and the patient following a tick bite. An 81-year-old woman presented with fever following a tick bite, with the tick still intact on her body. The patient was diagnosed with HGA. The tick was identified as Ixodes nipponensis by morphological and molecular biological detection methods targeting the 16S rRNA gene. The patient's blood was cultured after inoculation into the human promyelocytic leukemia cell line HL-60. A. phagocytophilum growth was confirmed via culture and isolation. A. phagocytophilum was identified in both the tick and the patient's blood by Anaplasma-specific groEL- and ankA-based nested polymerase chain reaction followed by sequencing. Moreover, a four-fold elevation in antibodies was observed in the patient's blood. We report a case of a patient diagnosed with HGA following admission for fever due to a tick bite. A. phagocytophilum was identified in both the tick and the patient, and A. phagocytophilum was successfully cultured. The present study suggests the need to investigate the possible incrimination of I. nipponensis as a vector for HGA in Korea.
Sections du résumé
BACKGROUND
BACKGROUND
Human granulocytic anaplasmosis (HGA) is a tick-borne infectious disease caused by Anaplasma phagocytophilum. To date, there have been no reported cases of A. phagocytophilum infection found in both the biting tick and the patient following a tick bite.
CASE PRESENTATION
METHODS
An 81-year-old woman presented with fever following a tick bite, with the tick still intact on her body. The patient was diagnosed with HGA. The tick was identified as Ixodes nipponensis by morphological and molecular biological detection methods targeting the 16S rRNA gene. The patient's blood was cultured after inoculation into the human promyelocytic leukemia cell line HL-60. A. phagocytophilum growth was confirmed via culture and isolation. A. phagocytophilum was identified in both the tick and the patient's blood by Anaplasma-specific groEL- and ankA-based nested polymerase chain reaction followed by sequencing. Moreover, a four-fold elevation in antibodies was observed in the patient's blood.
CONCLUSION
CONCLUSIONS
We report a case of a patient diagnosed with HGA following admission for fever due to a tick bite. A. phagocytophilum was identified in both the tick and the patient, and A. phagocytophilum was successfully cultured. The present study suggests the need to investigate the possible incrimination of I. nipponensis as a vector for HGA in Korea.
Identifiants
pubmed: 33176719
doi: 10.1186/s12879-020-05522-5
pii: 10.1186/s12879-020-05522-5
pmc: PMC7656494
doi:
Substances chimiques
Anti-Bacterial Agents
0
RNA, Ribosomal, 16S
0
Doxycycline
N12000U13O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
826Subventions
Organisme : Korea Centers for Disease Control and Prevention
ID : 2017-NI52002-00
Pays : Republic of Korea
Organisme : Korean Health Technology Research & Development Project, Ministry of Health and Welfare, Republic of Korea
ID : HI16C2118
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