Serum levels of miRNA-21-5p in vitiligo patients and effects of miRNA-21-5p on SOX5, beta-catenin, CDK2 and MITF protein expression in normal human melanocytes.
Adolescent
Adult
Case-Control Studies
Cell Line
Cell-Free Nucleic Acids
/ blood
Cyclin-Dependent Kinase 2
/ genetics
Gene Expression Regulation
Humans
Melanins
/ biosynthesis
Melanocytes
/ metabolism
MicroRNAs
/ blood
Microphthalmia-Associated Transcription Factor
/ genetics
Pilot Projects
SOXD Transcription Factors
/ genetics
Severity of Illness Index
Skin
/ cytology
Skin Pigmentation
/ genetics
Vitiligo
/ blood
Young Adult
beta Catenin
/ genetics
Melanocytes
SOX5
Vitiligo
miRNA
miRNA-21-5p
Journal
Journal of dermatological science
ISSN: 1873-569X
Titre abrégé: J Dermatol Sci
Pays: Netherlands
ID NLM: 9011485
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
05
05
2020
revised:
11
10
2020
accepted:
22
10
2020
pubmed:
13
11
2020
medline:
29
6
2021
entrez:
12
11
2020
Statut:
ppublish
Résumé
Epigenetics of vitiligo was evaluated in few studies. In particular, the role of miR-21, a microRNA involved in various processes, including melanogenesis, was never investigated. Evaluation of serum levels of miR-21-5p in vitiligo patients and miR-21-5p effects on melanogenesis. We measured serum levels of miR-21-5p in 40 patients affected by nonsegmental vitiligo and 40 sex- and age-matched healthy controls. Next, normal human melanocytes were transfected with miR-21-5p to study the effects of this microRNA, which targeted some proteins involved in melanogenesis pathway like SOX5, beta-catenin, cyclin-dependent kinase 2 (CDK2), and MITF. The expression of miR-21-5p in vitiligo patients was 3.6-4454.4 fold (mean 990.4 ± 1397.9) higher than in controls. The relative expression of miR-21-5p was directly and significantly correlated with disease severity, defined by VASI (Vitiligo Area and Severity Index) score (Rho = 0.89, p = 10 According to literature, miR-21-5p upregulation and consequent SOX5 downregulation should upregulate melanogenesis, while vitiligo is characterized by skin depigmentation. Our results suggest that current knowledge of the pathogenesis of vitiligo is probably incomplete. Clinical manifestations could result from an altered balance between metabolic pathways with contrasting effects. In this view, miR-21-5p upregulation might be a tentative compensation mechanism. Further studies appear necessary to confirm and better understand our results and their importance.
Sections du résumé
BACKGROUND
BACKGROUND
Epigenetics of vitiligo was evaluated in few studies. In particular, the role of miR-21, a microRNA involved in various processes, including melanogenesis, was never investigated.
OBJECTIVE
OBJECTIVE
Evaluation of serum levels of miR-21-5p in vitiligo patients and miR-21-5p effects on melanogenesis.
METHODS
METHODS
We measured serum levels of miR-21-5p in 40 patients affected by nonsegmental vitiligo and 40 sex- and age-matched healthy controls. Next, normal human melanocytes were transfected with miR-21-5p to study the effects of this microRNA, which targeted some proteins involved in melanogenesis pathway like SOX5, beta-catenin, cyclin-dependent kinase 2 (CDK2), and MITF.
RESULTS
RESULTS
The expression of miR-21-5p in vitiligo patients was 3.6-4454.4 fold (mean 990.4 ± 1397.9) higher than in controls. The relative expression of miR-21-5p was directly and significantly correlated with disease severity, defined by VASI (Vitiligo Area and Severity Index) score (Rho = 0.89, p = 10
CONCLUSION
CONCLUSIONS
According to literature, miR-21-5p upregulation and consequent SOX5 downregulation should upregulate melanogenesis, while vitiligo is characterized by skin depigmentation. Our results suggest that current knowledge of the pathogenesis of vitiligo is probably incomplete. Clinical manifestations could result from an altered balance between metabolic pathways with contrasting effects. In this view, miR-21-5p upregulation might be a tentative compensation mechanism. Further studies appear necessary to confirm and better understand our results and their importance.
Identifiants
pubmed: 33176966
pii: S0923-1811(20)30332-7
doi: 10.1016/j.jdermsci.2020.10.014
pii:
doi:
Substances chimiques
CTNNB1 protein, human
0
Cell-Free Nucleic Acids
0
MIRN21 microRNA, human
0
MITF protein, human
0
Melanins
0
MicroRNAs
0
Microphthalmia-Associated Transcription Factor
0
SOX5 protein, human
0
SOXD Transcription Factors
0
beta Catenin
0
CDK2 protein, human
EC 2.7.11.22
Cyclin-Dependent Kinase 2
EC 2.7.11.22
Types de publication
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
22-29Informations de copyright
Copyright © 2020 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors report no declarations of interest.