Impact of Laboratory Methods and Gene Targets on Detection of
Molecular Detection
Molecular Diagnostic Techniques
Real-Time Polymerase Chain Reaction
Streptococcus pneumoniae
blpA
Journal
Reports of biochemistry & molecular biology
ISSN: 2322-3480
Titre abrégé: Rep Biochem Mol Biol
Pays: Iran
ID NLM: 101637937
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
entrez:
12
11
2020
pubmed:
13
11
2020
medline:
13
11
2020
Statut:
ppublish
Résumé
Timely identification of A total of 440 clinical samples were collected from patients with suspected invasive pneumococcal infections during February 2016 to October 2018. Biochemical tests were used to confirm the dubious colonies on 5% sheep blood agar. Fifty-seven confirmed isolates, 57 culture-positive samples, and 57 culture-negative samples were analyzed for the presence of All the isolates and culture-positive samples were positive for The specificity of real-time PCR assay was significantly higher than that of conventional PCR for the identification of
Sections du résumé
BACKGROUND
BACKGROUND
Timely identification of
METHODS
METHODS
A total of 440 clinical samples were collected from patients with suspected invasive pneumococcal infections during February 2016 to October 2018. Biochemical tests were used to confirm the dubious colonies on 5% sheep blood agar. Fifty-seven confirmed isolates, 57 culture-positive samples, and 57 culture-negative samples were analyzed for the presence of
RESULTS
RESULTS
All the isolates and culture-positive samples were positive for
CONCLUSION
CONCLUSIONS
The specificity of real-time PCR assay was significantly higher than that of conventional PCR for the identification of
Identifiants
pubmed: 33178872
doi: 10.29252/rbmb.9.2.216
pii: rbmb-9-216
pmc: PMC7603256
doi:
Types de publication
Journal Article
Langues
eng
Pagination
216-222Références
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