Influenza A viruses limit NLRP3-NEK7-complex formation and pyroptosis in human macrophages.
NEK7
NLRP3
PB1-F2
influenza A virus
pyroptosis
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
03 12 2020
03 12 2020
Historique:
received:
12
03
2020
revised:
06
10
2020
accepted:
09
10
2020
pubmed:
13
11
2020
medline:
28
4
2021
entrez:
12
11
2020
Statut:
ppublish
Résumé
Pyroptosis is a fulminant form of macrophage cell death, contributing to release of pro-inflammatory cytokines. In humans, it depends on caspase 1/4-activation of gasdermin D and is characterized by the release of cytoplasmic content. Pathogens apply strategies to avoid or antagonize this host response. We demonstrate here that a small accessory protein (PB1-F2) of contemporary H5N1 and H3N2 influenza A viruses (IAV) curtails fulminant cell death of infected human macrophages. Infection of macrophages with a PB1-F2-deficient mutant of a contemporary IAV resulted in higher levels of caspase-1 activation, cleavage of gasdermin D, and release of LDH and IL-1β. Mechanistically, PB1-F2 limits transition of NLRP3 from its auto-repressed and closed confirmation into its active state. Consequently, interaction of a recently identified licensing kinase NEK7 with NLRP3 is diminished, which is required to initiate inflammasome assembly.
Identifiants
pubmed: 33180976
doi: 10.15252/embr.202050421
pmc: PMC7726813
doi:
Substances chimiques
Inflammasomes
0
NLR Family, Pyrin Domain-Containing 3 Protein
0
NEK7 protein, human
EC 2.7.11.1
NIMA-Related Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e50421Subventions
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF)
ID : SNF 310030_155949
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF)
ID : SNF 310030_155949/2
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF)
ID : SNF 310030_182475/1
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (SNF)
ID : 310030-185255
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : EXC 2155-390874280
Informations de copyright
© 2020 The Authors.
Références
Autophagy. 2005 Oct-Dec;1(3):174-6
pubmed: 16874072
Nature. 2019 Jun;570(7761):338-343
pubmed: 31189953
Nat Protoc. 2014 Nov;9(11):2663-81
pubmed: 25321410
PLoS Pathog. 2011 Jun;7(6):e1002099
pubmed: 21731493
EMBO Rep. 2020 Dec 3;21(12):e50421
pubmed: 33180976
J Invest Dermatol. 2015 Aug;135(8):2012-2020
pubmed: 25815426
J Virol. 2005 May;79(10):6441-8
pubmed: 15858027
Nat Immunol. 2011 May 29;12(7):631-8
pubmed: 21623379
J Gen Virol. 1990 Feb;71 ( Pt 2):477-9
pubmed: 2137872
Infect Immun. 2005 Apr;73(4):1907-16
pubmed: 15784530
Am J Respir Crit Care Med. 2014 Feb 15;189(4):449-62
pubmed: 24308446
J Leukoc Biol. 2017 Apr;101(4):863-874
pubmed: 27707881
J Virol. 2018 May 14;92(11):
pubmed: 29563290
Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8485-9
pubmed: 1924307
Gene. 1991 Dec 15;108(2):193-9
pubmed: 1660837
J Infect Dis. 2016 Dec 1;214(11):1658-1665
pubmed: 27601618
JCI Insight. 2017 Apr 6;2(7):e91868
pubmed: 28405622
Nat Med. 2001 Dec;7(12):1306-12
pubmed: 11726970
PLoS Pathog. 2011 Aug;7(8):e1002186
pubmed: 21852950
Eur J Immunol. 2015 Oct;45(10):2911-7
pubmed: 26174085
Sci Rep. 2016 Jun 10;6:27912
pubmed: 27283237
J Immunol. 1992 Aug 1;149(3):932-9
pubmed: 1321855
PLoS Pathog. 2013;9(5):e1003392
pubmed: 23737748
J Infect Dis. 2012 Dec 1;206(11):1685-94
pubmed: 22829640
Immunity. 2009 Apr 17;30(4):556-65
pubmed: 19362020
Immunol Res. 2016 Dec;64(5-6):1101-1117
pubmed: 27699580
PLoS One. 2012;7(3):e29879
pubmed: 22396727
Lancet Infect Dis. 2014 Jan;14(1):57-69
pubmed: 24239327
Nature. 2006 Mar 9;440(7081):228-32
pubmed: 16407890
J Immunol. 2008 Feb 15;180(4):2562-72
pubmed: 18250467
J Virol. 2009 Aug;83(16):8021-31
pubmed: 19494001
Proc Natl Acad Sci U S A. 2008 Jun 10;105(23):8067-72
pubmed: 18523012
Nat Commun. 2014 Aug 20;5:4713
pubmed: 25140902
Cell Res. 2016 Sep;26(9):1007-20
pubmed: 27573174
Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7119-24
pubmed: 19359475
J Leukoc Biol. 2012 Jul;92(1):11-20
pubmed: 22442495
Trends Biochem Sci. 2000 Mar;25(3):147-50
pubmed: 10694887
Proc Natl Acad Sci U S A. 2014 May 20;111(20):E2110-9
pubmed: 24799673
J Exp Med. 1996 Sep 1;184(3):1191-6
pubmed: 9064338
J Gen Virol. 2007 Feb;88(Pt 2):536-546
pubmed: 17251572
J Virol. 2009 Feb;83(4):1742-53
pubmed: 19073731
Nat Rev Mol Cell Biol. 2003 Feb;4(2):95-104
pubmed: 12563287
Trends Immunol. 2017 Apr;38(4):261-271
pubmed: 28196749
Immunobiology. 1993 Nov;189(3-4):340-55
pubmed: 8125516
Nat Med. 2006 Oct;12(10):1203-7
pubmed: 16964257
J Immunol. 2006 Aug 1;177(3):1817-24
pubmed: 16849492
mBio. 2018 Mar 20;9(2):
pubmed: 29559569
Nat Chem Biol. 2019 Jun;15(6):560-564
pubmed: 31086329
Nature. 2010 Dec 16;468(7326):968-72
pubmed: 21107320
J Gen Virol. 2012 Nov;93(Pt 11):2315-2325
pubmed: 22894921
J Leukoc Biol. 2012 Jul;92(1):1-4
pubmed: 22745457
PLoS Pathog. 2013 Feb;9(2):e1003188
pubmed: 23468627
Int J Mol Sci. 2017 Dec 29;19(1):
pubmed: 29286299
Nat Rev Immunol. 2010 Mar;10(3):210-5
pubmed: 20168318
Immunity. 2009 Apr 17;30(4):566-75
pubmed: 19362023
J Exp Med. 2009 Jan 16;206(1):79-87
pubmed: 19139171
Front Immunol. 2018 Apr 12;9:743
pubmed: 29755452
J Infect Dis. 2018 Jan 4;217(2):245-256
pubmed: 29112724
PLoS Pathog. 2010 Jul 22;6(7):e1001014
pubmed: 20661425
Nature. 2015 Oct 29;526(7575):666-71
pubmed: 26375259
Nat Immunol. 2016 Mar;17(3):250-8
pubmed: 26642356
Methods Mol Biol. 2018;1836:33-58
pubmed: 30151568
Am J Respir Crit Care Med. 2006 Nov 1;174(9):1011-7
pubmed: 16917113
PLoS Pathog. 2017 Jan 13;13(1):e1006140
pubmed: 28085958
Front Immunol. 2017 Oct 31;8:1420
pubmed: 29163496
J Exp Med. 2013 Sep 23;210(10):1977-92
pubmed: 24043763
Cell Host Microbe. 2007 Oct 11;2(4):240-9
pubmed: 18005742
Cell Microbiol. 2010 Jul;12(7):873-80
pubmed: 20482552
Trends Immunol. 2005 Aug;26(8):447-54
pubmed: 15967716
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
J Immunol. 1993 Nov 15;151(10):5574-85
pubmed: 8228247
Semin Immunol. 2009 Aug;21(4):194-8
pubmed: 19501527
Nature. 2015 Oct 29;526(7575):660-5
pubmed: 26375003
J Immunol. 1988 Apr 1;140(7):2267-73
pubmed: 3258333
J Biol Chem. 2006 Dec 1;281(48):36560-8
pubmed: 17008311
J Virol. 2016 Mar 28;90(8):4105-4114
pubmed: 26865721
Res Virol. 1996 Mar-Jun;147(2-3):123-30
pubmed: 8901431
J Biol Chem. 2016 Jan 1;291(1):103-9
pubmed: 26553871
Cell Mol Immunol. 2016 Jan;13(1):3-10
pubmed: 26189369
Ann N Y Acad Sci. 1998 Sep 29;856:1-11
pubmed: 9917859
Nat Immunol. 2010 May;11(5):404-10
pubmed: 20383149
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
J Biol Chem. 2016 Jan 8;291(2):739-51
pubmed: 26601953
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W22-8
pubmed: 23677614
Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):769-74
pubmed: 24367104
Nature. 2016 Feb 18;530(7590):354-7
pubmed: 26814970
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5227-31
pubmed: 2787508
PLoS Pathog. 2013;9(4):e1003256
pubmed: 23592984
Cytokine. 2019 Aug;120:115-124
pubmed: 31055218
J Gen Virol. 2012 Apr;93(Pt 4):840-849
pubmed: 22190016
J Virol. 2011 Dec;85(23):12324-33
pubmed: 21937639
J Exp Med. 2013 Mar 11;210(3):491-502
pubmed: 23460726
Annu Rev Immunol. 2009;27:519-50
pubmed: 19302047
J Biol Chem. 2017 Jan 20;292(3):826-836
pubmed: 27913620
J Virol. 2007 Mar;81(6):2736-44
pubmed: 17182684