Long-Term Maintenance of Acinar Cells in Human Submandibular Glands After Radiation Therapy.
Acinar Cells
/ pathology
Cell Plasticity
Cell Proliferation
/ radiation effects
Chemoradiotherapy
/ adverse effects
DNA Damage
Head and Neck Neoplasms
/ radiotherapy
Humans
Prospective Studies
Radiotherapy
/ adverse effects
Radiotherapy Dosage
Retrospective Studies
Submandibular Gland
/ drug effects
Vimentin
/ analysis
Journal
International journal of radiation oncology, biology, physics
ISSN: 1879-355X
Titre abrégé: Int J Radiat Oncol Biol Phys
Pays: United States
ID NLM: 7603616
Informations de publication
Date de publication:
15 03 2021
15 03 2021
Historique:
received:
18
05
2020
revised:
26
10
2020
accepted:
29
10
2020
pubmed:
13
11
2020
medline:
30
7
2021
entrez:
12
11
2020
Statut:
ppublish
Résumé
In a combined retrospective and prospective study, human salivary glands were investigated after radiation treatment for head and neck cancers. The aim was to assess acinar cell loss and morphologic changes after radiation therapy and to determine whether irradiated salivary glands have regenerative potential. Irradiated human submandibular and parotid salivary glands were collected from 16 patients at a range of time intervals after completion of radiation therapy (RT). Control samples were collected from 14 patients who had not received radiation treatments. Tissue sections were analyzed using immunohistochemistry to stain for molecular markers. Human submandibular and parotid glands isolated less than 1 year after RT showed a near complete loss of acinar cells. However, acinar units expressing functional secretory markers were observed in all samples isolated at later intervals after RT. Significantly lower acinar cell numbers and increased fibrosis were found in glands treated with combined radiation and chemotherapy, in comparison to glands treated with RT alone. Irradiated samples showed increased staining for duct cell keratin markers, as well as many cells coexpressing acinar- and duct cell-specific markers, in comparison to nonirradiated control samples. After RT, acinar cell clusters are maintained in human submandibular glands for years. The surviving acinar cells retain proliferative potential, although significant regeneration does not occur. Persistent DNA damage, increased fibrosis, and altered cell identity suggest mechanisms that may impair regeneration.
Identifiants
pubmed: 33181249
pii: S0360-3016(20)34483-7
doi: 10.1016/j.ijrobp.2020.10.037
pmc: PMC8061234
mid: NIHMS1689846
pii:
doi:
Substances chimiques
Vimentin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1028-1039Subventions
Organisme : NIDCR NIH HHS
ID : R56 DE025098
Pays : United States
Organisme : NIDCR NIH HHS
ID : UH3 DE027695
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Références
Dev Cell. 2015 Apr 20;33(2):231-7
pubmed: 25843887
Cell Rep. 2018 Aug 7;24(6):1464-1470.e3
pubmed: 30089258
Laryngoscope. 2005 Jun;115(6):958-64
pubmed: 15933500
Lancet Oncol. 2011 Feb;12(2):127-36
pubmed: 21236730
Nat Rev Clin Oncol. 2011 Jul 26;8(11):639-48
pubmed: 21788974
Radiother Oncol. 2011 Oct;101(1):93-9
pubmed: 21885141
Cell Tissue Res. 2020 Jun;380(3):487-497
pubmed: 31900666
Nat Rev Gastroenterol Hepatol. 2017 May;14(5):296-304
pubmed: 28270694
J Radiat Res. 2016 Aug;57 Suppl 1:i69-i75
pubmed: 27538846
Br J Cancer. 2001 Sep 28;85(7):1055-63
pubmed: 11592779
Oral Dis. 2015 Jan;21(1):e1-10
pubmed: 24581290
Oral Oncol. 2018 Nov;86:225-233
pubmed: 30409305
Crit Rev Oral Biol Med. 2003;14(3):199-212
pubmed: 12799323
J Histochem Cytochem. 2016 Jun;64(6):343-52
pubmed: 27126825
Wien Klin Wochenschr. 2017 Jun;129(11-12):398-403
pubmed: 28204968
Cancer Res. 2016 Mar 1;76(5):1170-80
pubmed: 26759233
Int J Radiat Oncol Biol Phys. 2005 Jul 1;62(3):659-64
pubmed: 15936542
Med Ultrason. 2013 Sep;15(3):173-9
pubmed: 23979611
FEBS Lett. 2020 Jan;594(2):376-382
pubmed: 31538335
Head Neck. 2016 Apr;38 Suppl 1:E1481-7
pubmed: 26561342
Int J Radiat Oncol Biol Phys. 2009 Mar 15;73(4):1088-95
pubmed: 18707823
Virchows Arch A Pathol Anat Histopathol. 1987;411(6):583-9
pubmed: 2445099
Oral Dis. 2019 Nov;25(8):1837-1839
pubmed: 31560148
Cancer Treat Rev. 2017 Sep;59:79-92
pubmed: 28759822
Virchows Arch. 2004 Apr;444(4):356-61
pubmed: 14762713
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 Sep;96(3):267-74
pubmed: 12973282
Biotech Histochem. 2008 Jun;83(3-4):103-30
pubmed: 18828044
Nat Rev Mol Cell Biol. 2014 Jan;15(1):7-18
pubmed: 24326623
Cancers (Basel). 2017 Oct 06;9(10):
pubmed: 28984841
Int J Radiat Oncol Biol Phys. 2001 Nov 15;51(4):938-46
pubmed: 11704314
Nature. 2018 May;557(7705):322-328
pubmed: 29769669
Genes Dev. 2017 Jan 15;31(2):154-171
pubmed: 28174210
Nat Rev Mol Cell Biol. 2016 Jul;17(7):413-25
pubmed: 26979497
Radiother Oncol. 2010 Oct;97(1):149-61
pubmed: 20888056
Cancer. 2006 Dec 1;107(11):2525-34
pubmed: 17078052
EMBO Mol Med. 2018 Mar;10(3):
pubmed: 29335337