Transformation of short-chain chlorinated paraffins by the bacterial haloalkane dehalogenase LinB - Formation of mono- and di-hydroxylated metabolites.
Biocatalysis
Environmental Monitoring
/ methods
Environmental Pollutants
/ analysis
Escherichia coli
/ enzymology
Halogenation
Hexachlorocyclohexane
/ analysis
Hydrocarbons, Brominated
/ analysis
Hydrocarbons, Chlorinated
/ analysis
Hydrolases
/ chemistry
Hydroxylation
Paraffin
/ analysis
Sphingomonadaceae
/ enzymology
Chlorinated olefins (COs)
Chlorinated paraffins (CPs)
Haloalkane dehalogenase LinB
Hydroxylated chlorinated paraffins
Persistent organic pollutants (POPs)
Journal
Chemosphere
ISSN: 1879-1298
Titre abrégé: Chemosphere
Pays: England
ID NLM: 0320657
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
27
05
2020
revised:
04
09
2020
accepted:
05
09
2020
entrez:
13
11
2020
pubmed:
14
11
2020
medline:
28
11
2020
Statut:
ppublish
Résumé
Short-chain chlorinated paraffins (SCCPs) are listed as persistent organic pollutants (POPs) under the Stockholm Convention. Such substances are toxic, bioaccumulating, transported over long distances and degrade slowly in the environment. Certain bacterial strains of the Sphingomonadacea family are able to degrade POPs, such as hexachlorocyclohexanes (HCHs) and hexabromocyclododecanes (HBCDs). The haloalkane dehalogenase LinB, expressed in certain Sphingomonadacea, is able to catalyze the transformation of haloalkanes to hydroxylated compounds. Therefore, LinB is a promising candidate for conversion of SCCPs. Hence, a mixture of chlorinated tridecanes was exposed in vitro to LinB, which was obtained through heterologous expression in Escherichia coli. Liquid chromatography mass spectrometry (LC-MS) was used to analyze chlorinated tridecanes and their transformation products. A chloride-enhanced soft ionization method, which favors the formation of chloride adducts [M+Cl]
Identifiants
pubmed: 33182101
pii: S0045-6535(20)32483-8
doi: 10.1016/j.chemosphere.2020.128288
pii:
doi:
Substances chimiques
Environmental Pollutants
0
Hydrocarbons, Brominated
0
Hydrocarbons, Chlorinated
0
Hexachlorocyclohexane
59NEE7PCAB
hexabromocyclododecane
5I9835JO3M
Paraffin
8002-74-2
Hydrolases
EC 3.-
haloalkane dehalogenase
EC 3.8.1.5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
128288Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.