Analysis of a persistent viral shedding patient infected with SARS-CoV-2 by RT-qPCR, FilmArray Respiratory Panel v2.1, and antigen detection.
Antigens, Viral
/ analysis
COVID-19
/ diagnosis
COVID-19 Testing
/ methods
Humans
Multiplex Polymerase Chain Reaction
/ methods
Nasopharynx
/ virology
RNA, Viral
/ analysis
Reverse Transcriptase Polymerase Chain Reaction
/ methods
SARS-CoV-2
/ immunology
Sensitivity and Specificity
Viral Load
Virus Shedding
Antigen
COVID-19
FilmArray
RT-qPCR
SARS-CoV-2
Journal
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy
ISSN: 1437-7780
Titre abrégé: J Infect Chemother
Pays: Netherlands
ID NLM: 9608375
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
09
08
2020
revised:
20
10
2020
accepted:
26
10
2020
pubmed:
14
11
2020
medline:
30
1
2021
entrez:
13
11
2020
Statut:
ppublish
Résumé
Various diagnostic tests utilizing different principles are currently under development for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, these tests can occasionally produce discrepant results, causing confusion in their interpretation. Here, we evaluated the performance and features of three diagnostic assays: quantitative reverse transcription polymerase chain reaction (RT-qPCR), FilmArray Respiratory Panel (RP) v2.1, and the LUMIPULSE antigen test. Twenty-seven serial nasopharyngeal swabs were collected from a prolonged viral shedding patient who had been hospitalized for 51 days. We examined the SARS-CoV-2 detection rates of the three tests. The overall agreement rate was 81% between RT-qPCR and FilmArray RP v2.1, 63% between the antigen test and FilmArray RP v2.1, and 59% between the antigen test and RT-qPCR. We obtained concordant results in samples with high viral loads (low threshold cycle values) by all three tests. RT-qPCR and FilmArray RP v2.1 accurately detected SARS-CoV-2 at the early to intermediate phases of infection, but the results varied at the late phase. The antigen test also produced a positive result at the early phase but varied at the intermediate phase and consistently produced negative results at late phase of infection. These results demonstrated FilmArray RP v2.1 could detect SARS-CoV-2 with accuracy comparable to RT-qPCR. Further, there were discrepant results using different types of diagnostic tests during the clinical course of prolonged viral shedding patient. We provided insights into how to utilize different types of kits to assess and manage SARS-CoV-2 infections.
Identifiants
pubmed: 33183963
pii: S1341-321X(20)30396-2
doi: 10.1016/j.jiac.2020.10.026
pmc: PMC7598429
pii:
doi:
Substances chimiques
Antigens, Viral
0
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
406-409Informations de copyright
Copyright © 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no competing interests.