CRISPR-Mediated Isogenic Cell-SELEX Approach for Generating Highly Specific Aptamers Against Native Membrane Proteins.
Affinity reagents
Aptamer
CRISPR
Nucleic acid therapeutics
SELEX
Journal
Cellular and molecular bioengineering
ISSN: 1865-5025
Titre abrégé: Cell Mol Bioeng
Pays: United States
ID NLM: 101468590
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
12
02
2020
accepted:
02
09
2020
entrez:
13
11
2020
pubmed:
14
11
2020
medline:
14
11
2020
Statut:
epublish
Résumé
The generation of affinity reagents that bind native membrane proteins with high specificity remains challenging. Most Using a Caco-2 epithelial cell line with constitutive Cas9 expression, we knocked out the 10 rounds of cell-SELEX were conducted Our data indicate that highly specific aptamers can be isolated with a SELEX strategy that utilizes isogenic cell lines. This approach may be broadly useful for generating affinity reagents that selectively bind to membrane proteins in their native conformations on the cell surface.
Identifiants
pubmed: 33184583
doi: 10.1007/s12195-020-00651-y
pii: 651
pmc: PMC7596163
doi:
Types de publication
Journal Article
Langues
eng
Pagination
559-574Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK058404
Pays : United States
Organisme : NCRR NIH HHS
ID : G20 RR030956
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY008126
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007347
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA068485
Pays : United States
Informations de copyright
© Biomedical Engineering Society 2020.
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