Reactive Fibroblasts in Response to Optic Nerve Crush Injury.
Animals
Cell Count
Cicatrix
/ pathology
Collagen Type I
/ metabolism
Crush Injuries
/ pathology
Extracellular Matrix
/ metabolism
Fibroblasts
/ pathology
Fibrosis
Macrophages
/ pathology
Mice, Transgenic
Microglia
/ pathology
Monocytes
/ pathology
Nerve Crush
Neuroglia
/ pathology
Optic Nerve
/ pathology
Optic Nerve Injuries
/ pathology
Pericytes
/ metabolism
Fibroblasts
Fibrotic scar
Optic nerve crush
Traumatic optic neuropathy
Collagen1α1 cells
Journal
Molecular neurobiology
ISSN: 1559-1182
Titre abrégé: Mol Neurobiol
Pays: United States
ID NLM: 8900963
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
18
05
2020
accepted:
03
11
2020
pubmed:
14
11
2020
medline:
28
10
2021
entrez:
13
11
2020
Statut:
ppublish
Résumé
Traumatic optic neuropathy leads to bidirectional degeneration of retinal ganglion cells and axons and results in optic nerve scaring, which inhibits the regeneration of damaged axons. Compared with its glial counterpart, the fibrotic response causing nerve scar tissue is poorly permissive to axonal regeneration. Using collagen1α1-GFP reporter mice, we characterize the development of fibrotic scar formation following optic nerve crush injury. We observe that perivascular collagen1α1 cells constitute a major cellular component of the fibrotic scar. We demonstrate that extracellular molecules and monocytes are key factors contributing to the pathogenesis of optic nerve fibrotic scar formation, with a previously unrecognized encapsulation of this scar. We also characterize the distribution of collagen1α1 cells in the retina after optic nerve crush injury based on in vivo and whole-mount retinal imaging. Our results identify collagen1α1 cells as a major component of fibrotic scarring following ONC and are a potential molecular target for promoting axonal regeneration after optic nerve injury.
Identifiants
pubmed: 33184784
doi: 10.1007/s12035-020-02199-4
pii: 10.1007/s12035-020-02199-4
pmc: PMC7933069
mid: NIHMS1646436
doi:
Substances chimiques
Collagen Type I
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1392-1403Subventions
Organisme : NEI NIH HHS
ID : P30 EY014801
Pays : United States
Organisme : NIH HHS
ID : P30EY014801
Pays : United States
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