Adenoviral E1A Exploits Flexibility and Disorder to Target Cellular Proteins.
CBP
E1A
IDP
NMR
fuzzy complex
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
11 11 2020
11 11 2020
Historique:
received:
15
10
2020
revised:
04
11
2020
accepted:
05
11
2020
entrez:
14
11
2020
pubmed:
15
11
2020
medline:
8
9
2021
Statut:
epublish
Résumé
Direct interaction between intrinsically disordered proteins (IDPs) is often difficult to characterize hampering the elucidation of their binding mechanism. Particularly challenging is the study of fuzzy complexes, in which the intrinsically disordered proteins or regions retain conformational freedom within the assembly. To date, nuclear magnetic resonance spectroscopy has proven to be one of the most powerful techniques to characterize at the atomic level intrinsically disordered proteins and their interactions, including those cases where the formed complexes are highly dynamic. Here, we present the characterization of the interaction between a viral protein, the Early region 1A protein from Adenovirus (E1A), and a disordered region of the human CREB-binding protein, namely the fourth intrinsically disordered linker CBP-ID4. E1A was widely studied as a prototypical viral oncogene. Its interaction with two folded domains of CBP was mapped, providing hints for understanding some functional aspects of the interaction with this transcriptional coactivator. However, the role of the flexible linker connecting these two globular domains of CBP in this interaction was never explored before.
Identifiants
pubmed: 33187345
pii: biom10111541
doi: 10.3390/biom10111541
pmc: PMC7698142
pii:
doi:
Substances chimiques
Adenovirus E1A Proteins
0
Intrinsically Disordered Proteins
0
CREB-Binding Protein
EC 2.3.1.48
CREBBP protein, human
EC 2.3.1.48
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fondazione Cassa di Risparmio di Firenze
ID : RP
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 19650
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