Ceramide ratios are affected by cigarette smoke but not heat-not-burn or e-vapor aerosols across four independent mouse studies.
Aerosols
/ adverse effects
Animals
Apolipoproteins E
/ genetics
Ceramides
/ analysis
Chromatography, Liquid
/ methods
E-Cigarette Vapor
/ adverse effects
Female
Humans
Lung
/ metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Proteomics
Risk Factors
Smoke
/ adverse effects
Tandem Mass Spectrometry
/ methods
Time Factors
COPD
Ceramides
Cigarette smoke
E-cigarettes
Heat-not-burn tobacco product
Lipidomics
Journal
Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521
Informations de publication
Date de publication:
15 Dec 2020
15 Dec 2020
Historique:
received:
20
08
2020
revised:
03
11
2020
accepted:
10
11
2020
pubmed:
16
11
2020
medline:
16
12
2020
entrez:
15
11
2020
Statut:
ppublish
Résumé
Smoking is an important risk factor for the development of chronic obstructive pulmonary disease and cardiovascular diseases. This study aimed to further elucidate the role of ceramides, as a key lipid class dysregulated in disease states. In this article we developed and validated LC-MS/MS method for ceramides (Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/24:0) and Cer(d18:1/24:1(15Z)) for the absolute quantification. We deployed it together with proteomics and transcriptomic analysis to assess the effects of cigarette smoke (CS) from the reference cigarette as well as aerosols from heat-not-burn (HnB) tobacco and e-vapor products in apolipoprotein E-deficient (ApoE In the lungs, CS exposure substantially elevated the ratios of Cer(d18:1/24:0) and Cer(d18:1/24:1) to Cer(d18:1/18:0) in two independent ApoE Our work in mice contributes to the accumulating evidence on the importance of ceramide ratios as biologically relevant markers for respiratory disorders, adding to their already demonstrated role in cardiovascular disease risk assessment in humans.
Identifiants
pubmed: 33189821
pii: S0024-3205(20)31506-X
doi: 10.1016/j.lfs.2020.118753
pii:
doi:
Substances chimiques
Aerosols
0
Apolipoproteins E
0
Ceramides
0
E-Cigarette Vapor
0
Smoke
0
Types de publication
Comparative Study
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
118753Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.