NanH and PknG putative virulence factors as a recombinant subunit immunogen against Corynebacterium pseudotuberculosis infection in mice.
CLA
Caseous lymphadenitis
Cellular response
Neuraminidase H
Protein kinase G
Journal
Vaccine
ISSN: 1873-2518
Titre abrégé: Vaccine
Pays: Netherlands
ID NLM: 8406899
Informations de publication
Date de publication:
03 12 2020
03 12 2020
Historique:
received:
06
05
2020
revised:
15
09
2020
accepted:
03
11
2020
pubmed:
17
11
2020
medline:
28
4
2021
entrez:
16
11
2020
Statut:
ppublish
Résumé
Despite the economic and zoonotic relevance of caseous lymphadenitis, a competent immunoprophylaxis tool is still necessary. Here, we evaluated two putative virulence factors of Corynebacterium pseudotuberculosis, rNanH, and rPknG, as recombinant subunit vaccines in a murine model against the infection by C. pseudotuberculosis. Three groups of ten Balb/c mice each were inoculated with a sterile 0.9% saline solution (G1), rNanH (G2), or rPknG (G3) in formulations containing saponin as an adjuvant. The mice received two vaccine doses intercalated by a 21-day interval and were challenged with 2 × 10
Identifiants
pubmed: 33190945
pii: S0264-410X(20)31436-5
doi: 10.1016/j.vaccine.2020.11.010
pii:
doi:
Substances chimiques
Bacterial Vaccines
0
Virulence Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8099-8106Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.