Plant miRNAs Reduce Cancer Cell Proliferation by Targeting MALAT1 and NEAT1: A Beneficial Cross-Kingdom Interaction.
MALAT1
NEAT1
cancer
long non-coding
nutrition
plant miRNA
Journal
Frontiers in genetics
ISSN: 1664-8021
Titre abrégé: Front Genet
Pays: Switzerland
ID NLM: 101560621
Informations de publication
Date de publication:
2020
2020
Historique:
received:
16
04
2020
accepted:
20
08
2020
entrez:
16
11
2020
pubmed:
17
11
2020
medline:
17
11
2020
Statut:
epublish
Résumé
MicroRNAs (miRNAs) are ubiquitous regulators of gene expression, evolutionarily conserved in plants and mammals. In recent years, although a growing number of papers debate the role of plant miRNAs on human gene expression, the molecular mechanisms through which this effect is achieved are still not completely elucidated. Some evidence suggest that this interaction might be sequence specific, and in this work, we investigated this possibility by transcriptomic and bioinformatics approaches. Plant and human miRNA sequences from primary databases were collected and compared for their similarities (global or local alignments). Out of 2,588 human miRNAs, 1,606 showed a perfect match of their seed sequence with the 5' end of 3,172 plant miRNAs. Further selections were applied based on the role of the human target genes or of the miRNA in cell cycle regulation (as an oncogene, tumor suppressor, or a biomarker for prognosis, or diagnosis in cancer). Based on these criteria, 20 human miRNAs were selected as potential functional analogous of 7 plant miRNAs, which were in turn transfected in different cell lines to evaluate their effect on cell proliferation. A significant decrease was observed in colorectal carcinoma HCT116 cell line. RNA-Seq demonstrated that 446 genes were differentially expressed 72 h after transfection. Noteworthy, we demonstrated that the plant mtr-miR-5754 and gma-miR4995 directly target the tumor-associated long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and nuclear paraspeckle assembly transcript 1 (NEAT1) in a sequence-specific manner. In conclusion, according to other recent discoveries, our study strengthens and expands the hypothesis that plant miRNAs can have a regulatory effect in mammals by targeting both protein-coding and non-coding RNA, thus suggesting new biotechnological applications.
Identifiants
pubmed: 33193626
doi: 10.3389/fgene.2020.552490
pmc: PMC7531330
doi:
Types de publication
Journal Article
Langues
eng
Pagination
552490Informations de copyright
Copyright © 2020 Marzano, Caratozzolo, Consiglio, Licciulli, Liuni, Sbisà, D’Elia, Tullo and Catalano.
Références
Nat Genet. 2005 May;37(5):495-500
pubmed: 15806104
BMC Genomics. 2015 Sep 03;16:676
pubmed: 26335021
Int J Clin Exp Pathol. 2015 May 01;8(5):5395-402
pubmed: 26191242
Genome Biol. 2014;15(12):550
pubmed: 25516281
Cell Res. 2012 Jan;22(1):107-26
pubmed: 21931358
PLoS One. 2012;7(3):e33663
pubmed: 22479426
Nucleic Acids Res. 2020 Jan 8;48(D1):D498-D503
pubmed: 31691815
J Nutrigenet Nutrigenomics. 2013;6(1):16-31
pubmed: 23445777
Cancer Res. 2015 Apr 1;75(7):1322-31
pubmed: 25600645
Crit Rev Food Sci Nutr. 2013;53(4):403-13
pubmed: 23320910
Cells. 2019 Nov 04;8(11):
pubmed: 31689969
RNA Biol. 2017 Dec 2;14(12):1705-1714
pubmed: 28837398
Genome Res. 2009 Jan;19(1):92-105
pubmed: 18955434
Nat Rev Mol Cell Biol. 2013 Aug;14(8):475-88
pubmed: 23800994
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Planta. 2018 Sep;248(3):545-558
pubmed: 29968061
Biomed Pharmacother. 2019 Mar;111:51-59
pubmed: 30576934
Oxid Med Cell Longev. 2015;2015:504253
pubmed: 26180591
Cancers (Basel). 2019 Feb 13;11(2):
pubmed: 30781877
Dev Biol. 2006 Jan 1;289(1):3-16
pubmed: 16325172
Nucleic Acids Res. 2002 Jan 1;30(1):38-41
pubmed: 11752248
Nutr Metab (Lond). 2018 Oct 1;15:68
pubmed: 30302122
Oncotarget. 2017 Apr 4;8(14):22783-22799
pubmed: 28187000
Mol Nutr Food Res. 2019 Jan;63(2):e1800076
pubmed: 30378765
Cell Res. 2016 Feb;26(2):217-28
pubmed: 26794868
Cell Host Microbe. 2018 Nov 14;24(5):637-652.e8
pubmed: 30449315
Nucleic Acids Res. 2017 Jan 4;45(D1):D1003-D1008
pubmed: 27580718
Nucleic Acids Res. 2010 Jan;38(Database issue):D5-16
pubmed: 19910364
Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9667-72
pubmed: 17535905
BMC Bioinformatics. 2011 Aug 04;12:323
pubmed: 21816040
Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3687-92
pubmed: 16505370
Cancer Manag Res. 2018 Dec 06;10:6757-6768
pubmed: 30584369
BMC Bioinformatics. 2016 Nov 8;17(Suppl 12):345
pubmed: 28185579
Cell. 2009 Jan 23;136(2):215-33
pubmed: 19167326
Genome Res. 2012 Sep;22(9):1760-74
pubmed: 22955987
Front Oncol. 2019 Jul 25;9:669
pubmed: 31404273
Nucleic Acids Res. 2009 Jan;37(1):1-13
pubmed: 19033363
Cell. 2005 Jan 14;120(1):15-20
pubmed: 15652477
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Proc Natl Acad Sci U S A. 2010 Sep 7;107(36):15751-6
pubmed: 20729470
Sci Rep. 2018 Aug 17;8(1):12413
pubmed: 30120339
PLoS Biol. 2005 Mar;3(3):e85
pubmed: 15723116
Nucleic Acids Res. 2011 Jan;39(Database issue):D163-9
pubmed: 21071411
Mol Cancer. 2017 Jun 14;16(1):104
pubmed: 28615056
Mol Nutr Food Res. 2014 Jul;58(7):1561-73
pubmed: 24842810
Planta. 2012 Dec;236(6):1875-87
pubmed: 22922939
Science. 2009 Nov 27;326(5957):1216-9
pubmed: 19965464
Nat Struct Mol Biol. 2018 Nov;25(11):1019-1027
pubmed: 30297778
Trends Genet. 2008 Jun;24(6):297-305
pubmed: 18450316
Nucleic Acids Res. 2000 Jan 1;28(1):27-30
pubmed: 10592173
Commun Biol. 2019 Aug 21;2:317
pubmed: 31453381
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D140-4
pubmed: 16381832
Cell. 2004 Jan 23;116(2):281-97
pubmed: 14744438
Genome Biol. 2010;11(8):R90
pubmed: 20799968
PLoS Genet. 2013 Mar;9(3):e1003368
pubmed: 23555285
Database (Oxford). 2018 Jan 1;2018:
pubmed: 29846545