Measuring the dynamic response of the thylakoid architecture in plant leaves by electron microscopy.
Arabidopsis thaliana
high‐pressure freezing
photosynthesis
stromal gap
thylakoid lumen
thylakoid membrane
transmission electron microscopy
Journal
Plant direct
ISSN: 2475-4455
Titre abrégé: Plant Direct
Pays: England
ID NLM: 101716131
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
05
08
2020
revised:
15
09
2020
accepted:
29
09
2020
entrez:
16
11
2020
pubmed:
17
11
2020
medline:
17
11
2020
Statut:
epublish
Résumé
The performance of the photosynthesis machinery in plants, including light harvesting, electron transport, and protein repair, is controlled by structural changes in the thylakoid membrane system inside the chloroplasts. In particular, the structure of the stacked grana area of thylakoid membranes is highly dynamic, changing in response to different environmental cues such as light intensity. For example, the aqueous thylakoid lumen enclosed by thylakoid membranes in grana has been documented to swell in the presence of light. However, light-induced alteration of the stromal gap in the stacked grana (partition gap) and of the unstacked stroma lamellae has not been well characterized. Light-induced changes in the entire thylakoid membrane system, including the lumen in both stacked and unstacked domains as well as the partition gap, are presented here, and the functional implications are discussed. This structural analysis was made possible by development of a robust semi-automated image analysis method combined with optimized plant tissue fixation techniques for transmission electron microscopy generating quantitative structural results for the analysis of thylakoid ultrastructure. A methodical pipeline ranging from optimized leaf tissue preparation for electron microscopy to quantitative image analysis was established. This methodical development was employed to study details of light-induced changes in the plant thylakoid ultrastructure. It was found that the lumen of the entire thylakoid system (stacked and unstacked domains) undergoes light-induced swelling, whereas adjacent membranes on the stroma side in stacked grana thylakoid approach each other.
Identifiants
pubmed: 33195966
doi: 10.1002/pld3.280
pii: PLD3280
pmc: PMC7644818
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e00280Informations de copyright
© 2020 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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