Immune response to cytosolic DNA via intercellular receptor modulation in oral keratinocytes and fibroblasts.
Antiviral Restriction Factors
/ immunology
Cell Line
Chemokine CXCL10
/ immunology
Cytoplasm
DNA, Viral
/ immunology
Fibroblasts
/ immunology
Humans
Immunity
Keratinocytes
/ immunology
Nuclear Proteins
/ immunology
Phosphoproteins
/ immunology
Receptors, Retinoic Acid
/ immunology
Simplexvirus
/ immunology
Tumor Necrosis Factor-alpha
/ immunology
IFI16
RIG-I
TNF-α
cytosolic DNA
oral fibroblasts
oral keratinocytes
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
revised:
31
10
2020
received:
05
05
2020
accepted:
02
11
2020
pubmed:
18
11
2020
medline:
25
12
2021
entrez:
17
11
2020
Statut:
ppublish
Résumé
Double-strand (ds) DNA-enveloped viruses can cause oral infection. Our aim is to investigate whether oral mucosal cells participate in immune response against cytosolic dsDNA invasion. We examined the response to transfected herpes simplex virus (HSV) dsDNA via intracellular receptors in oral keratinocytes (RT7) and fibroblasts (GT1), and the effect of TNF-α on those responses. Transfected dsDNA increased CXCL10 expression via NF-κB activation in both cell types, while those responses were inhibited by knockdown of RIG-I, an RNA sensor. Although IFI16, a DNA sensor, was expressed in the nuclei of both types, its knockdown decreased transfected dsDNA-induced CXCL10 expression in GT1 but not RT7 cells. IFI16 in GT1 cells was translocated into cytoplasm from nuclei, which was attributed to immune response to cytosolic dsDNA. TNF-α enhanced transfected dsDNA-induced CXCL10, and knockdown of IFI16 decreased TNF-α and dsDNA-driven CXCL10 expression in both RT7 and GT1 cells. Finally, the combination of TNF-α and transfected dsDNA resulted in translocation of IFI16 from nuclei to cytoplasm in RT7 cells. RIG-I and IFI16 in oral mucosal cells may play important roles in host immune response against DNA viral infection, while TNF-α contributes to development of an antiviral system via those intracellular receptors.
Substances chimiques
Antiviral Restriction Factors
0
CXCL10 protein, human
0
Chemokine CXCL10
0
DNA, Viral
0
Nuclear Proteins
0
PLAAT4 protein, human
0
Phosphoproteins
0
Receptors, Retinoic Acid
0
Tumor Necrosis Factor-alpha
0
IFI16 protein, human
148998-64-5
Types de publication
Journal Article
Langues
eng
Pagination
150-163Subventions
Organisme : Grant-in-Aid for Scientific Research from the Japan Society for Scientific Research (C) from the Ministry of Education, Culture, Sports, Science and Technology of Japan
ID : 17K11840
Organisme : Grant-in-Aid for Scientific Research from the Japan Society for Scientific Research (C) from the Ministry of Education, Culture, Sports, Science and Technology of Japan
ID : 26463010
Informations de copyright
© 2020 Wiley Periodicals LLC.
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