Antibacterial mechanism of Biochanin A and its efficacy for the control of Xanthomonas axonopodis pv. glycines in soybean.
Biochanin A
Xanthomonas axonopodis pv. glycines
antibacterial isoflavonoid
bacterial pustule disease
soybean
Journal
Pest management science
ISSN: 1526-4998
Titre abrégé: Pest Manag Sci
Pays: England
ID NLM: 100898744
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
revised:
29
10
2020
received:
29
07
2020
accepted:
17
11
2020
pubmed:
18
11
2020
medline:
18
3
2021
entrez:
17
11
2020
Statut:
ppublish
Résumé
Xanthomonas axonopodis pv. glycines (Xag) is a hazardous pathogen able to cause bacterial pustule disease in soybean, reducing crop yield and quality. Although flavonoids rutin and genistein are known to play an important role in soybean defence, soybean is only able to produce Biochanin A in low concentration. In this work, Biochanin A was found to produce higher antibacterial activity against Xag in comparison with genistein (minimum inhibitory concentration < 100 μg/mL). Biochanin A was able to inhibit DNA synthesis and flagella formation in Xag, and altered the composition of the bacterial membrane. These effects reduced swimming motility, extracellular protease activity and biofilm formation. Further, Biochanin A was tested for the control of Xag in soybean leaves, showing similar, or even higher, inhibitory ability in comparison with some products commonly used for the control of this pathogen. The antibacterial properties of Biochanin A against Xag have been studied for the first time, revealing new insights on the potential applications of this isoflavonoid for the management of bacterial pustule disease. © 2020 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Xanthomonas axonopodis pv. glycines (Xag) is a hazardous pathogen able to cause bacterial pustule disease in soybean, reducing crop yield and quality. Although flavonoids rutin and genistein are known to play an important role in soybean defence, soybean is only able to produce Biochanin A in low concentration.
RESULTS
RESULTS
In this work, Biochanin A was found to produce higher antibacterial activity against Xag in comparison with genistein (minimum inhibitory concentration < 100 μg/mL). Biochanin A was able to inhibit DNA synthesis and flagella formation in Xag, and altered the composition of the bacterial membrane. These effects reduced swimming motility, extracellular protease activity and biofilm formation. Further, Biochanin A was tested for the control of Xag in soybean leaves, showing similar, or even higher, inhibitory ability in comparison with some products commonly used for the control of this pathogen.
CONCLUSIONS
CONCLUSIONS
The antibacterial properties of Biochanin A against Xag have been studied for the first time, revealing new insights on the potential applications of this isoflavonoid for the management of bacterial pustule disease. © 2020 Society of Chemical Industry.
Substances chimiques
Anti-Bacterial Agents
0
Genistein
DH2M523P0H
Glycine
TE7660XO1C
biochanin A
U13J6U390T
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1668-1673Subventions
Organisme : China Postdoctoral Science Foundation
ID : 2018M642240
Organisme : Large Instruments Open Foundation of Nantong University
ID : KFJN2041
Organisme : Large Instruments Open Foundation of Nantong University
ID : KFJN2043
Organisme : Large Instruments Open Foundation of Nantong University
ID : KFJN2046
Organisme : Nantong Applied Research Program
ID : MS12017023-8
Organisme : National Natural Science Foundation of China
ID : 32050410290
Organisme : National Natural Science Foundation of China
ID : 81803407
Organisme : Natural Science Research Project of Jiangsu Higher Education Institutions
ID : 18KJB180023
Informations de copyright
© 2020 Society of Chemical Industry.
Références
Food and Agricultural Organization of the United Nations, 2018. Available: http://www.fao.org/faostat/en/#data/QC/visualize.
Machado EP, Dos S, Rodrigues Junior GL, Somavilla JC, Fuhr FM, Zago SL et al., Survival and development of Spodoptera eridania, Spodoptera cosmioides and Spodoptera albula (Lepidoptera: Noctuidae) on genetically-modified soybean expressing Cry1Ac and Cry1F proteins. Pest Manag Sci 76:4029-4035 (2020).
Hurley TM and Mitchell PD, The value of insect management to U.S. maize, soybean and cotton farmers. Pest Manag Sci 76:4159-4172 (2020).
Sartori FF, Pimpinato RF, Tornisielo VL, Engroff TD, Jaccoud DD, Menten JO et al., Soybean seed treatment: how do fungicides translocate in plants? Pest Manag Sci 76:2355-2359 (2020).
Chatnaparat T, Prathuangwong S and Lindow SE, Global pattern of gene expression of Xanthomonas axonopodis pv. glycines within soybean leaves. Mol Plant Microbe Interact 29:508-522 (2016).
Guo W, Gao J, Chen QS, Ma BJ, Fang Y, Liu X et al., Crp-like protein plays both positive and negative roles in regulating the pathogenicity of bacterial pustule pathogen Xanthomonas axonopodis pv. glycines. Phytopathology 109:1171-1183 (2019).
Guo W, Gao J, Wang HJ, Su RY, Sun CY, Gao SH et al., Phosphoglycerate kinase is involved in carbohydrate utilization, extracellular polysaccharide biosynthesis, and cell motility of Xanthomonas axonopodis pv. glycines independent of Clp. Front Microbiol 11:91 (2020).
Moon C, Seo DJ, Song YS and Jung WJ, Antibacterial activity of various chitosan forms against Xanthomonas axonopodis pv. glycines. Int J Biol Macromol 156:1600-1605 (2020).
Park H, Do E, Kim M, Park HJ, Lee J and Han SW, A LysR-type transcriptional regulator LcrX is involved in virulence, biofilm formation, swimming motility, siderophore secretion, and growth in sugar sources in Xanthomonas axonopodis pv. glycines. Front Plant Sci 10:1657 (2020).
Gómez JD, Vital CE, Oliveira MGA and Ramos HJO, Broad range flavonoid profiling by LC/MS of soybean genotypes contrasting for resistance to Anticarsia gemmatalis (Lepidoptera: Noctuidae). PLoS One 13:e0205010 (2018).
Algar E, Gutierrez-Mañero FJ, Garcia-Villaraco A, García-Seco D, Lucas JA and Ramos-Solano B, The role of isoflavone metabolism in plant protection depends on the rhizobacterial MAMP that triggers systemic resistance against Xanthomonas axonopodis pv. glycines in Glycine max (L.) Merr. cv. Osumi. Plant Physiol Biochem 82:9-16 (2014).
Hanski L, Genina N, Uvell H, Malinovskaja K, Gylfe A, Laaksonen T et al., Inhibitory activity of the isoflavone Biochanin A on intracellular bacteria of genus Chlamydia and initial development of a buccal formulation. PLoS One 9:e115115 (2014).
Sarfraz A, Javeed M, Shah MA, Hussain G, Shafiq N, Sarfraz I et al., Biochanin A: a novel bioactive multifunctional compound from nature. Sci Total Environ 722:137907 (2020).
Kole L, Giri B, Manna SK, Pal B and Ghosh S, Biochanin-A, an isoflavon, showed anti-proliferative and anti-inflammatory activities through the inhibition of iNOS expression, p38-MAPK and ATF-2 phosphorylation and blocking NFκB nuclear translocation. Eur J Pharmacol 653:8-15 (2011).
Wang L, Li LZ, Han QX, Wang XF, Zhao D and Liu JQ, Identification and biological evaluation of natural product Biochanin A. Bioorg Chem 97:103674 (2020).
Youn K, Park JH, Lee J, Jeong WS, Ho CT and Jun M, The identification of Biochanin A as a potent and selective beta-site app-cleaving enzyme 1 (Bace1) inhibitor. Nutrients 8:637 (2016).
Liang F, Cao W, Huang Y, Fang Y, Cheng Y, Pan S et al., Isoflavone Biochanin A, a novel nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element activator, protects against oxidative damage in HepG2 cells. Biofactors 45:563-574 (2019).
Jalaludeen AM, Ha WT, Lee R, Kim JH, Do JT, Park C et al., Biochanin A ameliorates arsenic-induced hepato- and hematotoxicity in rats. Molecules 21:69 (2016).
Elfiky AA, Natural products may interfere with SARS-CoV-2 attachment to the host cell. J Biomol Struct Dyn 38:1-10 (2020).
Weidenbörner M, Hindorf H, Chandra Jha H, Tsotsonos P and Egge H, Antifungal activity of isoflavonoids in different reduced stages on rhizoctonia solani and sclerotium rolfsii. Phytochemistry 29:801-803 (1990).
Prodana M, Bastos AC, Amaro A, Cardoso D, Morgado R, Machado AL et al., Biomonitoring tools for biochar and biochar-compost amended soil under viticulture: looking at exposure and effects. Appl Soil Ecol 137:120-128 (2019).
Park HJ, Jung B, Lee J and Han SW, Functional characterization of a putative DNA methyltransferase, EadM, in Xanthomonas axonopodis pv. glycines by proteomic and phenotypic analyses. Sci Rep 9:2446 (2019).
Chen GY, Zhang B, Wu XM and Zhao MQ, Cloning and characterization of an harpin-encoding gene from Xanthomonas axonopodis pv. glycines required for hypersensitive response on nonhost plant tobacco. Wei Sheng Wu Xue Bao 45:469-499 (2005).
Chen X, Sun C, Laborda P, Zhao Y, Palmer I, Fu ZQ et al., Melatonin treatment inhibits the growth of Xanthomonas oryzae pv. oryzae. Front Microbiol 9:2280 (2018).
Song XG, Han MH, He F, Wang SY, Li CH, Wu GC et al., Antifungal mechanism of dipicolinic acid and its efficacy for the biocontrol of pear valsa canker. Front Microbiol 11:958 (2020).
Carpenter SCD, Kladsuwan L, Han SW, Prathuangwong S and Bogdanove AJ, Complete genome sequences of Xanthomonas axonopodis pv. glycines isolates from the United States and Thailand reveal conserved transcription activator-like effectors. Genome Biol Evol 11:1380-1384 (2019).
Chen X, Sun C, Laborda P, He Y, Zhao Y, Li C et al., Melatonin treatments reduce the pathogenicity and inhibit the growth of Xanthomonas oryzae pv. oryzicola. Plant Pathol 68:288-296 (2019).
Du H, Huang Y and Tang Y, Genetic and metabolic engineering of isoflavonoid biosynthesis. Appl Microbiol Biotechnol 86:1293-1312 (2010).
Ulanowska K, Tkaczyk A, Konopa G and Wegrzyn G, Differential antibacterial activity of genistein arising from global inhibition of DNA, RNA and protein synthesis in some bacterial strains. Arch Microbiol 184:271-278 (2006).
Athinuwat D, Brooks S, Burr TJ and Prathuangwong S, Flagella and pili of Xanthomonasaxonopodis pv. glycines are associated with motility, biofilm formation and virulence on soybean. J Phytopathol 166:590-600 (2018).
Athinuwat D and Brooks S, The ompA gene of Xanthomonas axonopodis pv. glycines is involved in pathogenesis of pustule disease on soybean. Curr Microbiol 76:879-887 (2019).
Chatnaparat T, Prathuangwong S, Ionescu M and Lindow SE, XagR, a LuxR homolog, contributes to the virulence of Xanthomonas axonopodis pv. glycines to soybean. Mol Plant Microbe Interact 25:1104-1117 (2012).
Gottig N, Vranych CV, Sgro GG, Piazza A and Ottado J, HrpE, the major component of the Xanthomonas type three protein secretion pilus, elicits plant immunity responses. Sci Rep 8:9842 (2018).
Kim JG, Park BK, Yoo CH, Jeon E, Oh J and Hwang I, Characterization of the Xanthomonas axonopodis pv. glycines Hrp pathogenecity island. J Bacteriol 185:3155-3166 (2003).
He M, Wu T, Pan S and Xu X, Antimicrobial mechanism of flavonoids against Escherichia coli ATCC 25922 by model membrane study. Appl Surf Sci 305:515-521 (2014).