λ Recombineering Used to Engineer the Genome of Phage T7.
bacteriophage engineering
bacteriophage genetics
phage therapy
recombineering
Journal
Antibiotics (Basel, Switzerland)
ISSN: 2079-6382
Titre abrégé: Antibiotics (Basel)
Pays: Switzerland
ID NLM: 101637404
Informations de publication
Date de publication:
13 Nov 2020
13 Nov 2020
Historique:
received:
15
10
2020
revised:
10
11
2020
accepted:
12
11
2020
entrez:
18
11
2020
pubmed:
19
11
2020
medline:
19
11
2020
Statut:
epublish
Résumé
Bacteriophage T7 and T7-like bacteriophages are valuable genetic models for lytic phage biology that have heretofore been intractable with in vivo genetic engineering methods. This manuscript describes that the presence of λ Red recombination proteins makes in vivo recombineering of T7 possible, so that single base changes and whole gene replacements on the T7 genome can be made. Red recombination functions also increase the efficiency of T7 genome DNA transfection of cells by ~100-fold. Likewise, Red function enables two other T7-like bacteriophages that do not normally propagate in
Identifiants
pubmed: 33202746
pii: antibiotics9110805
doi: 10.3390/antibiotics9110805
pmc: PMC7697293
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIH HHS
ID : HHSN261200800001E
Pays : United States
Références
Proc Natl Acad Sci U S A. 1985 Oct;82(20):6783-7
pubmed: 2995984
Curr Protoc Mol Biol. 2007 Jul;Chapter 1:Unit 1.17
pubmed: 18265391
Biochim Biophys Acta. 1987 Jul 14;909(2):126-32
pubmed: 2439122
RNA Biol. 2014;11(1):42-4
pubmed: 24457913
Cell. 1983 Jun;33(2):315-7
pubmed: 6344999
J Mol Biol. 2004 Jan 30;335(5):1151-71
pubmed: 14729334
Cell. 2019 Oct 3;179(2):459-469.e9
pubmed: 31585083
Mol Microbiol. 2001 Apr;40(1):1-8
pubmed: 11298271
Mol Syst Biol. 2005;1:2005.0018
pubmed: 16729053
J Mol Biol. 2011 Mar 18;407(1):45-59
pubmed: 21256136
Curr Opin Microbiol. 2011 Oct;14(5):524-31
pubmed: 21868281
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6742-6
pubmed: 11381128
J Mol Biol. 1990 Dec 20;216(4):911-26
pubmed: 2266562
J Ind Microbiol Biotechnol. 2013 May;40(5):523-7
pubmed: 23478882
Genetics. 2010 Jan;184(1):221-32
pubmed: 19858285
Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1626-31
pubmed: 18230724
Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7267-72
pubmed: 26060300
Proc Natl Acad Sci U S A. 2006 Dec 12;103(50):19039-44
pubmed: 17135349
PLoS One. 2008;3(12):e3957
pubmed: 19088849
J Biol Chem. 1985 Mar 10;260(5):3197-206
pubmed: 4038707
Methods Mol Biol. 2009;501:239-51
pubmed: 19066825
Nucleic Acids Res. 2013 Dec;41(22):e204
pubmed: 24203710
Virology. 1969 Nov;39(3):562-74
pubmed: 4902069
Microbiol Rev. 1981 Mar;45(1):9-51
pubmed: 6261110
Cell Syst. 2015 Sep 23;1(3):187-196
pubmed: 26973885
mBio. 2016 Sep 13;7(5):
pubmed: 27624131
Genetics. 1998 Apr;148(4):1667-86
pubmed: 9560386
Science. 1972 Apr 28;176(4033):367-76
pubmed: 4554613
Nucleic Acids Res. 1995 Jun 11;23(11):1990-6
pubmed: 7596828
Curr Protoc Mol Biol. 2014 Apr 14;106:1.16.1-39
pubmed: 24733238
Virology. 2004 Feb 20;319(2):185-9
pubmed: 14980479
J Bacteriol. 1991 Sep;173(18):5808-21
pubmed: 1653221
FEMS Microbiol Lett. 2013 Jul;344(2):166-72
pubmed: 23651353