Proteomic analysis of extracellular vesicles reveals an immunogenic cargo in rheumatoid arthritis synovial fluid.
citrullination
extracellular vesicles
neutrophils
osteoarthritis
rheumatoid arthritis
synovial fluid
Journal
Clinical & translational immunology
ISSN: 2050-0068
Titre abrégé: Clin Transl Immunology
Pays: Australia
ID NLM: 101638268
Informations de publication
Date de publication:
2020
2020
Historique:
received:
10
12
2019
revised:
12
08
2020
revised:
08
09
2020
accepted:
09
09
2020
entrez:
18
11
2020
pubmed:
19
11
2020
medline:
19
11
2020
Statut:
epublish
Résumé
Extracellular vesicles (EVs) from rheumatoid arthritis (RA) synovial fluid (SF) have been reported to stimulate the release of pro-inflammatory mediators from recipient cells. We recently developed a size exclusion chromatography (SEC)-based method for EV isolation capable of high-quality enrichments from human SF. Here, we employed this method to accurately characterise the SF EV proteome and investigate potential contributions to inflammatory pathways in RA. Using our SEC-based approach, SF EVs were purified from the joints of RA patients classified as having high-level ( Synovial fluid EVs were present at higher concentrations in RA joints with high-level inflammation ( Our results provide new information about SF EVs and insight into how EVs might contribute to the perpetuation of RA.
Identifiants
pubmed: 33204424
doi: 10.1002/cti2.1185
pii: CTI21185
pmc: PMC7648259
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e1185Informations de copyright
© 2020 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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